In Vitro Antiangiogenic Activity Of Salvicine

OBJECTIVE: To study the antiangiogenesis activity of Salvicine on cell and molecular levels. METHODS: Modified MTT experiment, endothelial cell migration and capillary formation experiment were adopted to evaluate the effect of Salvicine on proliferation, migration and capillary formation of human microvascular endothelial cells (HMECs). RT-PCR was used to detect the VEGF165 and bFGF mRNA expression in human non-small cell lung adenocarcinoma cell line, A 549. RESULTS: Salvicine significantly inhibited the proliferation of HMECs, the IC50 value was 7.91 μ M; Salvicine strongly inhibited the migration of HMECs (P<0.05) at 1.25 μ M and the inhibition rate was 12.16%; Salvicine greatly inhibited capillary-like networks development of HMECs at the same concentration. Furthermore, bFGF mRNA expression level in A549 was significantly reduced after treatment of Salvicine at 30 μ M for 2 hours (P<0.05). But VEGF165 mRNA expression level inA549 unchanged at this point (P>0.05). CONCLUSION: By performing these in vitro angiogenesis assays, we concluded that Salvicine had a strong antiangiogenic effect on three important sequential angiogenic cascades: proliferation, migration and tube formation; Salvicine inhibited bFGF mRNA expression of A549 in vitro, which may be one of the mechanisms of antiangiogenesis of Salvicine.