Systemic Inflammation Induced By Lipoplysaccharide Increase Neointimal Formation After Balloon Injury In Porcine

Objective! The inflammation induced by the injury of artery plays akey role in the development of restenosis after percutaneous transluminal coronary angioplasty or stent implantation. Increasing researches show systemic inflammation probably influences the formation of restenosis also. The aim of the present study was toelucidate this problem. Method : Twelve adult miniswines were randomly divided into two groups. One group(n=6) was injected bacterial lipopolysaccharide(6 y g/swine)before balloon injury and the other group was injected saline as control. Iliac artery balloon injury was performed to all the animals. A systemic marker of inflammation(serum tumor necrosis factor- a levels)was measured by ELISA before this procedure and four, twenty four hours later to evaluate the degree of inflammation. Four weeks after the injury morphometric analysis was performed on injured arterial segments and normal iliac artery to measure the neointimal area, the area of lumen, internal elastic lamina and percent stenosis. Results:(l) Four hours after injury, the concentration of serum TNF- a increased in all animals.(2)Four weeks later, both neointimal formation and negative remolding contribute to restenosis in control group.(3)the neointimal area and the degree of stenosis in LPS-treated animals were increasedcompared with controls but the negative remolding was notobserved.Conclusions I Inflammation immediately after arterial injury promotes neointimal formation and luminal loss.