| Objective: To evaluate the cell compatibility of ZrO2-CBC by studying the influence of ZrO2-CBC on the growth of osteoblasts derived from SD fetal rats in vitro.Materials and Methods: Primary osteoblasts were cultured from SD fetal rats' calvaria and then were identified, purified and passaged. ZrO2-CBC (as experiment group) and pure titanium (as control group) were prepared to be round thin slice (thickness 1.0mm, diameter 15.0mm) and then cleaned and sterilized for spare use. After that, both materials and osteoblasts were co-cultured in vitro for 15 days to evaluate the effect of the materials on cells in Id, 5d, 10d and 15d, respectively. Results: Cells derived from SD fetal rats' calvaria were determined to be osteoblasts with higher purity and activity. It was found that ZrO2-CBC did not prevent the growth of osteoblasts at the earlyphase (P>0.05 ) , but it restrained the cells' proliferation and ALP secretions atthe later phase (P<0.05) , compared with control group. Osteoblasts hadadhered to the surface of the material and begun to grow since the 1st day ofco-culture. The cells on the surface continued to increase and stretched into thedeep part of ZrO2-CBC.Conclusion: It was proved in vitro that ZrO2-CBC did no visible harm toosteoblasts derived from SD fetal rats' calvaria. It is a promising dental implantmaterial.
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