Effects Of Sodium Ferulate On The Experimental Colitis Of Rats And Its Mechansm

Sodium ferulate (SF) is one of the effective components of Angelica sinensis. Many researches have revealed that SF possesses plenty of beneficial pharmacological effects such as inhibiting arachidonic acid metabolis, inhibiting macrophages activation and platelets activation, improving endothelial function, improving microcirculation relieving oxidative stress. Furthermore, SF almost have no toxicity. Basis researches had proved that SF could ameliorated immunological injury in some diseases mainly caused by immunity factors. We presumed that SF might be a possible candidate to be used for the treatment of IBD, which to our knowledge has not been investigated in previous studies. The present study was therefore conducted to confirm our hypothesis with an effort to demonstrate the therapeutic effects of sodium ferulate on experimental colitis.On the basis of establishment of two colitis modelof rats induced by acetic acid or trinitrobenzene sulfonic acid with ethanol respectively, We firstly observe the protective effect of sodium ferulate by enema on experimental colitis of rats, then investigate its relative mechanism at different level using different methods. We believe this work would be helpful to provide some valuable evidence for application of sodium ferulate against inflammatory bowel disease. The protocol of this research would be carried out as follows: to evulate effects of sodium ferulate on experimental colitis model of rats induced by acetic acid and investigate its mechanism; to evulate effects of sodium ferulate on experimental colitis model of rats induced by trinitrobenzene sulfonic acid with ethanol and investigate its mechanism. The experimental animals were randomly divided into normal control, model control, and three dose SF therapy groups, and treated intracolonically with normal saline, normal saline, and SF 200, 400 and 800 mg kg-1 respectively and daily for 7 days 24 h following the induction of colitis. After rats treated with acetic acid or trinitrobenzene sulfonic acid with ethanol respectively, observation of colitis symptoms and some indicators reflecting degree of colon damage in rats are more severe than that in normal group markably which show these models are accomplished successfully. After treated with sodium ferulate by enema, the colon mucosa damage index, histopathological score, inflammation indexes and oxidant indexes were evaluated.The results of this reseach showed that administration of SF intracolonically had protective effect on the colon injury in the rat model of colitis induced by acetic acid enema, which was probably due to the mechanism of antioxidation, inhibition ofarachidonic acid metabolis and platelets activation, administration of SF intracolonically also showed protective effect on the colon injury in the rat model of colitis induced by trinitrobenzene sulfonic acid and ethanol, which was probably due to antioxidation and inhibition of macrophages activation.