The Influence Of Sodium Butyrate On Colon Carcinoma Cells And The Expression Of Inducible Nitric Oxide Synthase

Objective : It was proved that .Sodium Butyrate can inhabit the growth of many cancer cell lines in vitro.HT-29 cell line is a cell line which is used in numerous researchs about colon cancer. Our study was investigate the effect of Sodium Butyrate on the growth of HT-29 colon carcinoma cells, the expression of iNOS and the excretion of NO from the cells to find out the mechanism of butyrate to colon cancerMETHODS: HT-29 cell lines were cultured in the medium of 10% calf blood serum, 100IU/ml penicillin, 100ug/ml streptomycin and in the environment of 37℃ 5%CO2 saturation degree of wetness for multiplicative growths transfer of culture and reserve. Sodium Butyrate in various concentrations of 1.0 mmol/L, 2.0 mmol/L,5.0 mmol/L,10.0 mmol/L and 20.0 mmol/L was co-incubated with the cells, then adopted MTT test to detect the cells' proliferation inhibitory concentration of 50% cells(IC50). and stained the iNOS protein of HT-29 cells with a immunochemical method (SP Staining).The absorbance value(A value) of iNOS was measured by the system of automatic image analysis .Griess's technique was used to detect the excretion of nitric oxide(NO).RESULTS: the survival rates of HT-29 cells treated by Sodium Butyrate decreased in a time-dependant and dose-dependent manner. IC50) value of Sodium Butyrate at different treatment periods are altered(15.4 mmol/L at 12 hours. 5.7 mmol/L at 24 hours. 2.5 mmol/L at 36 hours, 0.9 mmol/L at 48 hours). Simultaneously Sodium Butyrate decreased the expression of iNOS in the cells" cytoplasm and the excretion of NO in the similar manner.CONCLUSION: Sodium Butyrate could inhibit the expression of iNOS, consequently reduce the excretion of NO. It maybe one of the mechanisms by which Sodium Butyrate inhibits the growth of HT-29 colon carcinoma cells.