The Effect Of Selective Digestive Decontamination In Sever Acute Pancreatitis In Rats

Objective: To explore the effect of selective digestive decontamination (SDD) to decrease bacterial translocation and relieve inflatmmatory response in sever acute pancreatitis (SAP) in rats.Methods: The Sprague-Dawley rats were divided randomly into norm group, sham-operated group, SAP group, SDD group. SAP was induced by intraductal injection of 4% sodium taurocholate. SDD group underwent bacterial decontamination through the administration of Gentamycin, Polymyxin and Nystatin during the 3 days prior to SAP induction. 24 hours after SAP induction, laparotomy was performed and samples for the bacterial culture of pancreas, lung, liver, spleen and kidney was obtained. Intestinal bacteria was counted. A point scoring system of histologicalal features was used to evaluate the severity of pancreatitis. The levels of blood endotoxin in portal vein and plasma TNF alpha in venae cavae were measured. Pancreatic myeloperoxidase (MPO) were detected at the same time.Results : In the SAP and SDD group, there was significant pancreatic and extrapancreatic infection 24 hours after induction of pancreatitis, counting of intestinal bacteria, plasma concentrations of endotoxin and TNF alpha were significantly higher than in norm group and sham-operated group controls. Compared with SAP group, all of above-mentioned indexes were decreased in SDD group.Conclusions The incidence of bacterial translocation and the severity of SAP can decreased by SDD prior to SAP induction.