| Chronic cerebral hypoperfusion is chronic cerebrovascular insufficiency for a long time because of all kinds of reasons. It brings out histopathological and biochemical changes in brain ,which is a common pathological way in the period of many disease such as vascular dementia . Binswanger disease . Alzheimer disease .With the ratio of senile increasing in our country, prevention and treatment to diseases mainly from chronic cerebrovascular insufficiency is increasingly important. In the situation of cerebral hypoperfusion, pathological changes take place in nerve synapse, especially in cholinergic synapses. At the same time, free oxidate increases, SOD, its clearer, discreases, LPO increases with its toxic output -MDA increasing relatively. MDA is a kind of toxic matter, which can overoxidate cell membrane. Astrocytes increased, GFAP, their symbol protein, increased too. Tanakan is standard extract of ginkgo biloba. The main ingredient is ginkgolide and bilobalide. The research indicates that tanakan can improve cerebrovascular cycle, improve energy metabolism, clear free oxidate, protect the intact of structure and ability of the cell membrane. Therefore ,it protects the neurons in hypoperfusion .The experiment research the protective effect of tanakan to cerebral hypoperfusion by observing microstructure of synapse . SOD. MDA. GFAP to support prevention and therapy senile diseases with tanakan.Materials and methods: (1) Selected 80 Wistar rats and distributed to 4 groups4randomly, regardless of male or female, 20 rats each group. Group A: controlled (sham operation plus solvent); group B: only treated (sham operation plus tanakan plus solvent); group C: only hypoperfusion (hypoperfusion plus solvent); group D: hypoperfusion and treatment (hypoperfusion plus tanakan plus solvent). Rats underwent permanent bilateral occlusion of the common carotid arteries (2VO) in groups C and D. Bilateral common carotid arteries were only isolated but not ligated in groups A and B. groups A and C swallowed 0.5% carboxymethyl cellulose sodium solution 3ml, groups B and D swallowed the same amount of tanakan (200mg/mg) disolved by 0.5% carboxymethyl cellulose sodium solution. All the rats after were raised in a usual way for 7 weeks, were decapitated. Hippocampus (1 1 2mm)of the rats used to be observed were taken out, then put into solution of 4% glutaral, after a series of treatments, insected to 50nm thick, observed them under 80 KV H-7500 electrolen. The rats used to detect S MDA were decapitated and taken out brains ,then grilled brains and made 10% solution, at last detect SO MDA . The rats used to immunohistochemical observed were shot saline in brain arties then shot with 4% polyformaldehyde solution to be fixed, sacrificed by decapitation, the brain tissue was removed, embed in paraffin and made immunohitochemical sections(3um thick).Using HE staining and immuniohistochemical SABC method, the number of GFAP -positive cells were detected. The data was handled with SPSSIO.O statistic software. The difference of every two groups was compared with one-way analysis of variance and LSD method, significant level is a =0.05.Results: (1). All the rats were in low spirits, reacted slowly, ate little after operation. The next day, sham groups recovered in spirits, reacted quickly and ate more, but hypoperfusion groups improved insignificantly in the period of treatment.(2). Synapes of groups A, B are good observed by electrolen, no swell at membranes of synapses and no blur in gaps between synapses .But in group C, the membranes of synapses are swollen, the gaps between synapes are blurry and matter increased at the promembrane. The membranes of synapses of group D are insignificantly swollen.(3). SOD: group A: 151.58+6.79 ; group B: 156.90+6.02; group C: 123.71 ?.11; group D: 134.49 + 8.15. MDA: group A: 5.35?.67; group B: 5.14?.58; group C: 12.92?.87; group D: 8. 14 ?.73. The difference is significant (P<0.01) between every two groups except groups A and B in SOD and MDA, which indicated that tanakan has n... |
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