| Objectives:To evaluate whether the chemosensitivity test of peripherl blood lymphocytes of esophageal carcinoma and cardiac carcinoma can replace the chemosensitivity test of tumor cells in vitro to guide clinical individual chemotherapy. In addition, to study the relationship between the multidrug resistance-1gene expression and the vitro chemosensitivity test. Chemotherapy possess an impotant status in the all-around therapy of esophageal carcinoma and cardiac carcinoma as operation. But there is no pertinence in current experiential chemotherapy. In the recent years, the study that the chemosensitivity assay guilds clinical individual chemotherapy is attached importance to by doctors.Vivo or vitro drug sensitivity assay which applies on human fresh tissue is good for applying drug, foreasting drug sensiyivity to individual tumor cells, instructing clinic, improving curative effect. Research in this aspect has been carried through for many years. The common assays are coloration eliminating, fluorescence detecting, human tumor cloning, biochemistry assay and subrenal capsule xenograft assay . There are some disadvantages in the above assays. Firstly, experimental period is too long. Secondly, experimental operations are hard. Finally, it is difficult to acquire the samples. Therefore, it is an exigent task to find out a kind of sample to replace tumor tissue. The peripheral blood lymphocyte is also an antipersonnel object in chemotherapy. It has been reported that relativity between the peripheral blood lymphocyte and tumor cells has been approved in vitro chemosensitivity assay. The purpose of our research is to estimate relativity between the peripheral blood lymphocyte and tumor cells .In addition, it is an important factor that the expression of multidrug resistance gene leads to the failure of chemotherapy. Many scientists have also carried through the research. But the relationship between the multidrug resistance gene expression and vitro chemosensitivity test in esophageal carcinoma and cardiac carcinoma is rarely investigated. We not only studied the significance of P-plycoprotein which acts as classical sign of multidrug resistance in esophageal carcinoma and cardiac carcinoma, but also analysed the relationship between the multidrug resistance gene expression and vitro chemosensitivity test. The study will be helpful in the clinical individual chemotherapy of esophageal carcinoma and cardiac carcinoma.Methods: With MTT Assay in vitro, we determined the chemosensitivity of nine kinds of drug which used to be applied in clinic. And we analysed the relationship of the chemosensivity between the peripheral blood lymphocyte and the tumor cells of human esophageal carcinoma and cardiac carcinoma. Moreover, we evaluated the expression of P-plycoprotein in the primary tumor tissue, the surrounding noncancerousous tissue, metastatic lymph nodes and nonmetastatic lymph nodes by flow cytometry and immunofluorescence technique. In order to study the expression of P-plycoprotein, we collected respectively five normal organic cell samples of both oesophagus and cardia as comparison.Results: ⑴ It was showed that the tumor cells of esophageal carcinoma were sensitive to 5-Fu, DDP, CBCDA, VP16 and MTX. The rate of sensitivity to chemotherapeutic drugs were 40.6% ( 5-Fu ), 36.1% ( DDP ), 33.6% ( CBCDA ), 31.3%(VP-16 ), 31.2% ( MTX ). Squamous cell carcinoma was sensitive to 5-Fu, DDP, CBCDA, MTX and VP16. The rate of sensitivity to chemotherapeutic drugs in other pathology genres has no statistical significance. ⑵ It was showed that the tumor cells of cardiac carcinoma were sensitive to CBCDA, VP16, MTX, THP, 5-Fu. The rate of sensitivity to chemotherapeutic drugs were 34.80% (CBCDA), 32.47% (VP16), 31.60% (MTX), 31.60% (THP), 30.50%(5-Fu). Adenocarcinoma was sensitive to ADM, DDP, CBCDA, THP and 5-Fu. The rate of sensitivity to chemotherapeutic drugs in other pathology genres has no statistical significance. ⑶ There was no statistical difference between the rate of sensitivity to the...
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