The Effect Of Morhine On The Proliferation Of Uterus Endometrial Cells And ERmRNA Expressed In These Cells.

Objective: To study the effect of morphine in estrogen-induced proliferation of cultured human uterus endometrial stromal and epithelial cells and ERmRNA expressed in these cells. Method: Isolate and culture human epithelial and stromal cells in vitro. They were identified by immunocytochemical staining. Observe the effect of morphine in 17-βE2 -induced proliferation of the epithelial and stromal cells by cell growth curve and study the ERmRNA expressed in these two types of cells by RT-PCR tecknique.Result: 1. Enzymolysis and filtration were available to isolate, purify human epithelial and stromal cells. Epithelial cells have tadpole appearance and grow in whorls. They were stained positively by CK19. Stromal cells were spindle or polygonal shape. They were stained positively by vimentin. 2.17-βE2 induce proliferation of the epithelial and stromal cells. Morphine inhibit the effect of estrogen(P﹤0.05) and opioid peptide antagonist nalotone retort the effect of morphine. 3.17-βE2 increase the expression of ERmRNA and morphine decrease it(P﹤0.05) and nalotone retort the effect of morphine. The inhibiting action of morphine depend on its dose in stromal cells ,r=-0.946. Conclusion : Morphine inhibit the estrogen-induced proliferation of human epithelial and stromal cells and the reason probably is it decreased the expression of ERmRNA in above two types of cells.