| Cerebral atherosclerosis is one of the basic reasons of Cerebral Vessels Accidental Accession. But because its occurrence and development involves a long process and is affected by many factors, the factors that are found to attribute to brain atheromatous fleck under experimental conditions fail to be assured in human body. Moreover, the interaction between various factors is so complex that the pathogenesis of AS has not been completely specified. Researchers have proposed many theories or hypotheses, some of which have been eliminated. Up to date, the theories of the pathogenesis of AS fall into two categories: one is that the response to damaging factors of vessels endothelium activate the AS fleck (Response to injury); the other is that the commence element of AS fleck may be the genetic mutation of smooth muscle (Monoclonal Theory). Commonly believed by scholars is that five substances play important roles in the formation of AS, i.e. vessel smooth muscle cell,Vessel endothelium cell, platelet, lipoprotein and monocyte. The integrity of the endothelium cell's structures and functions is necessary to the maintenance of vessel system's structures and functions. The Blood Stream Protective Barrier hypothesis was found by Zhuang Minghua of this research group when he was investigating the conformation of vessel endothelium of Chinese children'. He found that the cerebral cortex capillary vessel was bark-like and the cortex medulla vessels stretched at an obtuse angle into the cortex. The hypothesis claims that human beings are born with the conformation of unsmooth vessel endothelium. Under the physiological condition, the blood stream forms a protective barrier on the unsmooth surface of the vessel endothelium so that the physiological smoothness helps to maintain normal flow of blood. This experiment is aimed at further testifying the functions of blood stream protective barrier in the pathogenesis and Treatment of cerebral vessels accidental Accession and providing theoretical evidence for cerebral vessels accidents.Adjectives:! To investigate the functions of blood stream in the pathogenesis of cerebral atherosclerosis and to verify the existence of blood stream protective barrier and it's changes in the vivid cerebral atherosclerosis pathological model.2 To provide theoretical evidence for the pathogenesis of cerebral atherosclerosisthrough observation of blood stream's changes in the pathological model after the usage of sodium ferulate and ahalysantinfarctasum to improve the rheology of the blood.3. To provide experimental evidence for the treatment of cerebral atherosclerosis through simulated treatment of cerebral atherosclerosis by using sodium ferulate &ahalysantinfarctasum in the pathological model to observe the blood stream changes.Methods:1. Facture of animal model: 30 grown-up male big-ear rabbits were provided by the Experimental Animal Department of Guangzhou Chinese Medicine University (certificate's number : 2001A067 )average weight=2.0kg-3.0kg, which were randomly divided into control group, pathological group and preventive group. Rabbits in the control group were normally fed. Those in the pathological group were daily fed with cholesterol (Ig), lard (3g) and yolk (Ig) in their feedstuff At the same time, they were given injection of ox serum albumin (250mg/kg) for immune damage on the vessel wall. The rabbits in the preventive group were fed similar to those in the pathological group for 4 weeks. But at the same time, they were treated with sodium ferulate& ahalysantinfarctasum for preventive purpose.2. Observation through confocal laser scanning microscope: First, symbolize the blood with fluorescein sodium. Then scan the optical slices on the continuous faultage of the same section on the parietal operculum of the rabbit model (scanning depth=250-300um). Measure the caliber of the vessels and, according to different groups, investigate the changes of the blood stream's thickness and conformation when the cerebral atherosclerosis happened. In this way, we monitored t...
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