The Effect Of Intra-Arterial Influsion Chemotherapy On Apoptosis And Proliferation In Cervical Cancer

Objective To investigate the effect of intra-arterial influsion chemotherapy on apoptosis and proliferation in cervical cancer. Methods Cervical tissues in normal cervix group, cervical cancer group before intra-arterial influsion chemotherapy and after intra-arterial influsion chemotherapy were examined, each group involved 30 cases. Apoptosis index (AI) was detected by DNA in situ terminal-deoxynucleotidyl transferase mediated nick end labeling (TUNEL). Ki67 labeling index (LI) was detected by SP immunohistochemical technique. The rate of AI/LI was analyzed.Results1. Apoptosis index (AI) in cervical cancer group before intra-arterial influsion chemotherapy(5.77(0.97)and after intra-arterial influsion chemotherapy(11.65(2.36)were higher than that in normal cervix group (2.54(0.87,). AI in cervical cancer group before intra-arterial influsion chemotherapy was significantly lower than that after intra-arterial influsion chemotherapy (p<0.05). 2. Ki67 labeling index (LI) in cervical cancer group before intra-arterial influsion chemotherapy(53.93(20.82)and after intra-arterial influsion chemotherapy(18.13(11.61)higher than that in normal cervix group (13.03(4.76,P<0.05). LI in cervical cancer group before intra-arterial influsion chemotherapy was significantly higher than that after intra-arterial influsion chemotherapy (P<0.05).3. The rate of AI/LI in cervical cancer group before intra-arterial influsion chemotherapy lower than that in normal cervix group, the rate of AI/LI in cervical cancer group after intra-arterial influsion chemotherapy higher than that in normal cervix group. The rate of AI/LI in cervical cancer group after intra-arterial influsion chemotherapy higher than that before intra-arterial influsion chemotherapy (P<0.05).Conclusion Interventional chemotherapy can induce apoptosis of human cervical cancer cells and inhibit the proliferation . The rate of AI/LI can express the balance of cell apoptosis and proliferation.