| Objective: To explore the best suitable experiment condition used to compare the MTS colorimetry and the 3H-TdR incorporation. To compare the difference of MTS colorimetry and 3H-TdR incorporation in the drug sensitivity test, sequentially to definitude the feasibility of using MTS colorimetry to examine tumor drug sensitivity and the clinic application foreground . Methods: 1. exerted different concentration liver cancer SMMC-7721 cell plant to process 3H-TdR incorporation tumor drug sensitivity test, to find out the best suitable cell concentration used to carry through 3H-TdR incorporation tumor drug sensitivity test, and compared with the suggesting cell concentration of the MTS colorimetry, then confirmed the best suitable experiment cell concentration ; 2. determined the liver cancer SMMC-7721 cell plant restraining rate under the action of different concentration anticancer drug(5-FU) used the 3H-TdR incorporation,then Confirmed the best suitable experiment drug concentration through comparing them. 3. determined the liver cancer SMMC-7721 cell plant restraining rate of the best suitable cell concentration under the action of anticancer drug (5-FU) of the best suitable drug concentration using the 3H-TdR incorporation, compared the difference of cell restraining rate when incorporate the 3H-TdR in different time, then determined the best suitable time of incorporating 3H-TdR. 4. Used parallely 3H-TdR incorporation and MTS colorimetry to measure the liver cancer SMMC-7721 cell plant and the stomach cancer NKM-45 cell plant restraining rate under the action of clinical anticancer drugs such as: ADM,5-FU,DDP,BLM,IFO,MTX,VP-16,NVB,CPT,MMC. Then compared the relativity of the two tumor drug sensitivity test. Results: 1. The CPM value through 3H-TdR incorporation has positivity correlation with cell quantity when the cell concentration is between 1×103/well- 4×104/well, r=0.9837(p<0.001). compared the suitable cell concentration through 3H-TdR incorporation drug sensitivity test with the suggestion concentration of the MTS colorimetry, the author adopted 1×104/well as experiment cell concentration. 2. It restrained middly the liver cancer SMMC-7721 cell plant of 1×104/well cell concentration when the drug (5-FU) concentration was 0.25×PPC, but it resrained highly the cells of the same concentration when the drug (5-FU) concentration was between 0.5×PPC to 5.0×PPC, the author adopted 1.0×PPC as experiment drug concentration basing on the experiment result and clinic feasibility. 3. measured the CPM value of the test group (adding drug group) and the contrast group (no adding drug group) when incorporated 3H-TdR in different time (4h-24h before gathering the cells). The CPM values of contrast group is obviously relative to the time of incorporating 3H-TdR, r=0.964(P<0.001). the author adopted the time of 8h before gathering cells as 3H-TdR incorporating time basing on the experiment results and work advantage principle. 4. there was no significant difference between the two group when the author measured the sensitivity of SMMC-7721 liver cancer plant and NKM-45 stomach cancer plant to 10 clinical drug using 3H-TdR incorporation and MTS colorimetry(P>0.05). The SMMC-7721 cell plant was highly sensitive to DDP,ADM,5-FU,CPT; and it was lowly sensitivity to MTX,VP-16,MMC,NVB; but it was not sensitive to BLM,IFO. The NKM-45 cell plant was highly sensitive to NVB; middly sensitive to ADM,5-FU,CPT,MMC; and it was lowly sensitive to VP-16; but it was not sensitive to DDP ,BLM,IFO,MTX. Conclusion: MTS colorimetry is a new tetrazolium salt compound, the author found out the sensitivity level is close between MTS colorimetry and 3H-TdR incorporation, but the MTS colorimetry is easily operated, rapid,and has no isotope pollution, so it has wide clinical appliance foreground in the tumor drug sensitivity test field.
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