The Proliminary Study On Human Knee Meniscal Replacement Made By Tissue Engineering Technique

Menisci are thought to be very important in load bearing, load distribution, shock absorption, joint lubrication and stabilization of the knee joint[1.2.3]. It is very common of human meniscus injury and discoid meniscus deformation in department joint surgery[4]. All these injuries and deformation need to be resected by surgical precedure[4.5]. Though these measures can ease the symptoms after operations, there are still some complications left, such as degeneration of meniscus. Nowadays, the available treatment measures on these injuries and deformation still have deficiencies. The technique in tissue engineering provides a new approach for the meniscus injury and discoid meniscus deformation treatment. The advantages include:(1) the engineered meniscus posses biological activity;(2)the source of the seeding cell can easily got in the patients by arthroscopy;(3)the shape of the tissue engineered meniscus can be selected meet to the patient need. (4)the antigenicity is not obvious if the seeding cell come from the autos. By present,there have been many initial problems to be solved, such as optimal accumulation of seeding cells, preparation of biodegradable scaffold composites, the actions of growth factors, three-dimensional culture of cell-scaffold complex, efficiency of the tissue engineered meniscus to repair the injuried and deformal meniscus. It predicts that the time of "from the organ trnansplant to organ copy"is coming as the research of tissue engineering has been developed quickly. Many important problems must be sovled for repairing meniscus in clinic by bioengineering technology. Our study attempt to research human meniscus fibro-chondroncyte as seeding cell and reconstruction tissue engineered meniscus in vivo of nude mouse to repair the meniscus injury and meniscus discoid deformation.contents1.The optimal accumulation of meniscus fibro-chondrocyte for meniscus tissue engineering.2.The study of the biological characteristics of meniscus fibro-chondrocyte in vitro.3.The reconstruction of the tissue engineered human meniscus in vivo of nude mouse.Result1.the meniscus tissue from the operation can be used as the source of meniscus tissue engineering seeding cell[14]. The harvest of seeding cell is average of (356±25)×104 per gram wet weigh meniscus tissue.There is no differerce between the age's ranges from 10 to 90 years old.2.The best seeding density of meniscus fibrochondrocyte is from 0.4×104/cm2 to 4×104/cm2 in spite of the primary or passage culture cells. But the primary generation fibrochondrocyte grows much slower than that of the passage generation 's. 3.the growth curve of the meniscus fibrochondrocyte shows that there is a slight descend the second day after seeding, but when the time on the fibrochondrocyte grows faster and faster until arriving the "platform period " which the fibrocytes stays at for about 3 to 4 days, and then the curve descend sharply. 4.Some kinds of biological characteristics of the meniscus have been investigated in this study such as the anchoring rate, the express of collagen type ⅠmRNA stimulated by the growth factors and the collagen typeⅠand type Ⅱ detection of immunological histology test and so on.5.The tissue engineered human meniscus shows some the same biological characteristis as the normal human meniscus.conclusion1.The meniscus tissue from the operation can be used as one of the most important sources of the seeding cell for the meniscus tissue engineering.2.The meniscus fibrochondrocyte's biological characteristics show that the third to the fifth generation of the fibrochondrocyte is the best seeding cell for meniscus tissue engineering. 3.The reconstruction of tissue engineered human meniscus is feasible.the tissue engineered human meniscus shows many aspects the same characteristics as the normal humam meniscus.