Expression And Clinical Significance Of Multidrug Resistance Gene In Ovarian Tumors

ObjectiveTo explore the gene expression of multidiig resistance gene(MDR1) in ovarian normal tissue or ovarian tumor tissue and its clinical significance.To provide theoretical groundwork for chemotherapy of ovarian cancer.MethodsThe fluorogenic quantitative Reverse transcription polymerase chain reaction(FQ-RT-PCR) was used to determine the expression of MDRi ovarian carcinomar(34cases), benign mmor(30cases) and normal tissue(20cases). the expression level of MDRi and the association between this gene and chemotherapy result. ResultThe expression rate of MDRi gene in malijpiant,benign tumor and normal tissue of ovary was 100%, 33.3%, and 0% respectively. The expression level of MDRi in malignant rumor(M206.05 X 104copy/ml) was greater than benign rumor(MOcopy/ml)r P<0.001. There were significant differences between histologyical subtypes(serious carcinoma, mucious carcinoma), hiatologyical grading and disease stage(FIGO stage I, II /III, IV), P<0.05,MDRIPO.05-, PO.001. The level of MDRi in patients with half life of CAi25>25days was 2.048 X 107 copy/ml , but the level of MDRi in patients with halflife of CA]25 <16 days was 2.724X106 ccpy/ml .Association was foundbetween the expression level of MDRi and chemotherapy result, PO.05.ConclusionExpression of MDRi is one of major factors associated with drug-resistancein ovarian carcinoma.