Animal Test Investigation Of ～(125)I Seeds Brachytherapy To Treat Malign Glioma

OBJECTIVE Malign glioma is a familiar encephalic tumors.Because most malign glioma are invasive,operation results are not ideal,and radiotherapies are often needed to boost operations.125I seeds have been widely used in many abroad countries and have partly replaced routine radiotherapies because of their good therapy results.But the validity and feasibility of 125I seeds' small dosage is not known.This investigation that has observed the tumor growth curve,HE dyeing,propidium iodide (PI) dyeing follow cytometry check,p53 and TERT immunohistochemical dying,wants to seach after the proper dosage to 125I seeds' brachytherapy and expatiate the mechanisms of them.METHODS 24 mice were divided to 4 groups with random principle:when the tumor diameter reach 11 mm (0.7ml),the comparison group without therapy;0 mCi group,0.4m Ci group and 0.8 mCi group were implanted by 0 mCi,0.4 mCi and 0.8 mCi 125I seeds individually in the centre of the tumor.Observing the tumor growth curve,HE dyeing,propidium iodide (PI) dyeing follow cytometry check,p53 and TERT immunohistochemical dying in order to seach after the proper dosage to 125I seeds' brachytherapy and expatiate the mechanisms of them.CONCLUSIONS (1) Planting C6 malign glioma to Balb/c female nude mice's endermic tissue is a good animal model to research of anoxic glioma's long-term therapy.(2) Once tumor cells overstep the dosage bound,and receive the stimulation dosage,they will be difficult to control.(3) By the outcome of animal investigation in vivo,small 125I seeds' dosage is in effect. We recommend that 125I seeds' dosage should be 6~12Gy (>24Gy is not necessary) and should add radiotherapy and chemical therapy to brachytherapy in time.