An Experimental Study On FK506 And CyclosporinA In Rabbit's Corneal Epithelium Wound Healing

Objective To investigate the effect of tacrolimus (FK506)in rabbit's corneal epithelial wound healing .To study the mechanism of FK506 by the expression of epidermal growth factor (EGF )and fibronection (Fn )in corneal epithelial wound healing .and compared with cyclosporin A(CsA) Methods Corneal epithelial wound model of 18New Zealand white rabbits was made .The 18 rabbits were divided into 3groups ,Every groups were random divided into 4 and 2 rabbits .Each right eye of the 4 rabbits were dosed with 0.1%FK506,The left eye with 0.5%CsA.The 2 animals being treated with no eye drops .Three groups were photographed every 0, 12, 24, 36, 48, 72hours spectively after being dosed. Histopathological and immunohistochemical examination on corneas were performed at 3.5,4,5 days after making Corneal epithelial wound model. To statistical analysis . Results Q-test showed statistically significant difference (p < 0.05 = between 0.1%FK506 group and the other groups .there was no significant difference between 0.5%CsA group and control group.The expression of EGF decreased gradually in the FK506 group after 3.5,4,5days .the difference is significant between the 5 and 3.5days (p<0.01).And the expression of Fn decreased gradually in FK506, the difference is significant between the 5 and 3.5days (p<0.05).The differences of the CsA, the control groups are nosignificance . Conclusions Time until rabbit's corneal epithelium wound healing is significantly shorter with 0.1%FK506 compared with the control and the model 0.5%CsA given, and 0.5%CsA is not. The expression of EGF and Fn decreased in FK506 group would largely contribute to its helpful of corneal epithelial wound healing.