The Study Of GnRH Receptor In Normal Endometrium And Endometrial Cancer By Hybridization In Situ

GnRH receptor is composed of 327-328 amino acid, have 7 step membrane structural region, belong to G protein On unite by body, with other G protein On unite by body Difference is, should lack the C end broken structural region in cell by body, human GnRH receives body gene to locate in chromosome 4 q13, 2-13, On 3 is from 3 besides show son With 2 contain the only copy gene that son forms, its 5 flank districts have the district of nucleic acid of a 700 bp, has 5 TATA boxes Concerning different gene transcribe initial point with other breast-feed kind of animal compare, the transcribe point of start bit of person is in More the location of upper reaches, show that GnRH receptor the body gene transcribe possible existent kind of start bit to belong to peculiarness. Traditional viewpoint is considered, promote the Gonadotropin-releasing hormone (GnRH) is from the hypothalamic hormone, controlling the secretion of both FSH and LH from the anterior pituitary. GnRH that pass hypothalamy pulse system with the way of pulse secrete anterior pituitary. GnRH receptor make it secrete to promote the hormone of sexual gland: LH and FSH, rear two take affect in sexual gland , produce peculiar sex hormone, such as: In female stimulate ovary secrete estradiol and progesterone, in male stimulate testosterone and prostaglandin. Secrete high factor for breast gland and adrenal gland thyroid gland and skeleton growth play role. In last few years concerning the basic research of GnRH, it is clinical to apply, and The GnRH research such as thing is just unfolding. Think with traditional viewpoint different, some scholars, pituitary outside normal organization existent GnRH receptor such as: Cerebrum Mammary gland, ovary, testis, prostate, placenta organization, and there is also high affinity in the malignant tumor organizations such as breast cancer, ovary cancer and prostate cancer GnRH receive body express. Test to adopt hybridization in situ from mRNA horizontal proof GnRH receive body the membrane in normal endometrium and endometrial carcinoma express. This experiment uses paraffin section In which secrete the membrane in 30 endometrial cancer example and womb in 23 normal hyperplasia endomtrium example, 30 membrane example and normal hyperplasia issue womb in issue womb normally 3 not typical hyperplasia examples, and take 3 examples Use brain pituitary organization do positive compare, it is compared that PBS replaces probe to do negative. Experiment result shows, in 30 examples secrete the membrane organization in issue womb normally, GnRH mRNA that receives body express 5/30 example, in normal hyperplasia issue womb, membrane is organized In GnRH mRNA that receives body express 4/23, and in the membrane cancer organization in womb GnRH mRNA that receives body express 12/30 example. In womb membrane not typical hyperplasia In organization, 3 is regular to not see to express. In a word, our research result shows that in normal endocrium and endocritriol cancer have GnRH receptor to receive mRNA of body to express, just In the endocritial cancer and in normal endocrium, GnRH receives mRNA of body to express, have discrepancy (p<0.05). And is secreting issue normally in the membrane in membrane and hyperplasia issue womb in womb nonsignificant difference (p>0.05). What special needs point out is that test only proof GnRH receives body, in pituitary outside organization in express besides, more hope the membrane in womb and the membrane in normal womb Through GnRH, the membrane carninomatosis in not typical hyperplasia and womb changes course, gets the condition that body mRNA expresses, find some possible hints, but what regret is that because of In womb membrane not typical hyperplasia case number lose, and without a regular positive, express, so have no conclusion of this respect.