The Research Of Curative Effect And Mechanism On Anti-Hepatic Fibrosis Interfered By RouganyixianYin

Object Observe the curative effect and the advantage caused by Rougany ixianYin in terms of anti-hepatic fibrosis, and reveal the mechanism of it.Methods Clinic study: 60 patients with early hepatocirrhosis are treated with RouganyixianYin for 6 months. Before and after treatment, test the indexes of hepatic function and the contents of fibrosis including HA, LN, PC III and VI-C in serum, and observe the show of liver and spleen in Doppler. Experimental research: The rats in which hepatic fibrosis model was induced by carbon tetrachloride (CCL4) were divided into three groups. Except the model and control groups, the prevention and therapy groups all received RouganyixianYin in different period for 8 weeks by poured in. After all rats were put into death, tested the indexes of hepatic function and the contents of HA, LN, PC III in fibrosis and SOD, MDA in peroxidation in serum. The histopathology of hepatic tissue was observed by histological examination and Masson' s dye which were performed in hepatic tissue, and immunohistochemical assay which the expression of Desmin was measured by, so we can comprehend the changes of denaturation of hepatic cells, hyperplasia of collagen and the activation of hepatic stellate cells in terms of semi-quantity in all groups.Results After counted with statistics, there were vary amendments in clinic symptoms, hepatic function, the indexes of fibrosis and the show ofliver and spleen in Doppler of 60 patients, and the discrepancies compared between before and after treatment were obvious (p<0.05 or P<0.01). The indexes in serum and the semi-quantities of denaturation of hepatic cell, hyperplasia of collagen and the activation of HSC in hepatic tissue of treatment groups had better amendments than those in model group, and the discrepancies compared among them are significant. The effect in therapy group was better than that in prevention group.Conclusion RouganyixianYin has accurate curative effects, including the protection of hepatic cells, the depression of collagen' s hyperplasia and the antagonism of hepatic stellate cell' s activation. So it can ameliorate the symptoms and the indexes tested in lab of patients.