| 1. By improving surgery technique, to try to create a stable and applied model on ectopia whole small intestine transplantation of rat, namely, tri-cuff vascular anastomosis technique2. Referring to classical induction program, to try to separate dentritic cell and progenitors from the rat bone marrow and then culture and amplify them to become immature dendritic cell3.Using the donor's dendritic cell derived from the rat bone marrow to pre-treat recipient, reproduce rat small intestine transplantation, observe the recipient's general condition and transplant bowel's alive time after transplantation, detect rejection' grade of the transplant bowel.Methods The experiment has three portions.1. SBT portion: 60 cases adult Wistar rats were randomly divided into two groups as recipients: experiment group and control group; another 60 cases adult Wistar rats were made as donors, and then did Wistar allograft SBT. Experimental rats underwent tri-cuff vascular anastomosis technique, while control rats underwent classical vascular anastomosis technique, then the results of the two groups were compared with each other.2. adopting direct separation and induce method: based on the classical method with GM-CSF+IL-4, we restrained rat dendritic cell derived from bone marrow from immature to mature adding IL-10 to the culture medium on the 5th day. During the cultivation, we observed the DC's form with light microscope and detect the DC's phenotypes with flow cytometry.Direct separation and induce method: with low osmotic pressure to splitting erythrocyte, with special T and B cell monoclonal antibodies to destruct T , B Lymphocytes , macrophage and other cells, then Utilizing the weak adhesiveness of dendritic cells, separate the dendritic cells from the blood or bone marrow, then culturing and amplifying the dendritic cells with adding the cytokine to the culture medium3. 24 cases adult SD rats were randomly divided into four groups as recipients: control group, CsA group, immature DC group and CsA+ immature DC group, 6 cases each group. Another 24 cases adult Wistar rats were made as donors, control group rats underwent no pretreatment, while CsA group rats underwent CsA, 15mg/kg, im,l time a dayx5days(after operation); immature DC group rats underwent injection of vein with the immature dendritic cells(lxl06/rat) cultured above from the Wistar rats, while the CsA+ immature DC group rats underwent injecting CsA+ immature DC.After pretreatment, all four group rats were given SBT, Wistar SD on the 8th day. During post-transplantation, recipients' general conditions and transplant bowels' alive time were observed and compared with each other. After transplantation on the 3th, 5th, 7th day, transplant intestine mucous membrane was taken and did tissue pathological inspection.Results1. The part of the SBT(1).30 cases rats in the experiment group had underwent SBT. 27cases were successful and 3 cases failed, operation success rate was 90% . Average arteriovenous anastomosis time of the recipient was 8? mins, transplant bowel thermo-ischemia averagely was 10?mins, operation time spent on the recipient averagely was 60?0mins, all operation time spent on the recipient and donor averagely was 120?0mins30 cases rats in the control group had underwent SBT. 24cases were successful and 6 cases failed, operation success rate was 80%. Average arteriovenous anastomosis time of the recipient was 38? mins, transplant bowel thermo-ischemia averagely was 22?mins, operation time spent on the recipient averagely was 100?5mins, all operation time spent on the recipient and donor averagely was 170?0mins(2). There were significant difference between two groups on the average arteriovenous anastomosis time transplant bowel thermo-ischemia time and operation time spent on the recipient (P<0.01) .(3).There were no significant difference between two groups on the operation success rate complication rate and changes on the body weight of the recipient after transplantation (P>0.05) .2. The part of the imDC cultiva...
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