Studies On HPA Genotyping And Its Clinical Significance

Objective:To established HPA genotyping method and explored platelet transfusion refractoriness.Methods:1. According to difference of HPA gene, 5 pairs of oligonucleotide specific primers for HPA gene were designed and composed. The polymerase chain reaction sequence specific oligonucleotide primer (PCR-SSP) was used to amplify and detect the HPA gene.2. The methods of microcolumn gel test and Capture-p were used in screening and identification of HPA antibodies.3. HPA gene polymorphism in Hefei's donor was investigated and platelet donor regirster was founded.4. Platelet-reactive antibodies in patients of platelet transfusion refractoriness were detected, and compatible donors were selected for platelet transfusion.Result:1. The method of HPA genotyping by PCR-SSP had high sensitivity .specificity and repetition.2. The highest frequencies of HPA genes was HPA -4a (0.993) in Hefei's donor and the second was HPA-la (0.973). The lowest frequencies of HPA genes was HPA -4b (0.007) in Hefei's donor, and the second was HPA-lb (0.027). At the same time, we established the platelets donor register.3. In 32 cases of platelet transfusion refractoriness, 6 cases resulted from HPA antibody. Of them, 4 cases were HPA-la, 2 case was HPA-5b.-4-4. Present study showed that platelets transfusion was successful by selecting HPA compatible donors.Conclusion:A method of genotyping was developed for HPA-1, -2, -3 and -5 by means of the PCR amplification with sequence-specific primers (PCR-SSP) technique. Primer sets were designed to allow PCR amplification for all systems using the same assay conditions. Specificity, sensitivity and repetition of the method are good.The HPA register can be established by investigation of HPA polymorphism in Hefei's donor and it can overcome PTR that result from HPA antibodies.