| BackgroudChemotherapy induced alopecia (CIA) is one of the common side effects of many chemotherapeutic drugs, which reduces the patients' compliance for chemotherapy. Therefore, how to prevent CIA is one of the fundamental problems in clinical chemotherapy.Chemotherapy is commonly used in clinic for cancer treatment, which often has side effects, such as alopecia. Hair follicles grow in cycles from phases of rapid growth (anagen), via regression (catagen) to relative quiescence (telegen). The sensitivity of hair follicle cells to anticancer agents is related to their state of proliferation. In anagen stage, the hair follicle matrix cells in bulb are highly proliferating and sensitive to chemotherapeutic drugs.It has been thought that CIA is because of chemotherapeutic drugs induced apoptosis of hair follicle cells in anange stage, which blocks normal maturation of precursor epithelial cells and early precipitates the follicles into catagen stage. Many approaches have been used for preventing CIA, such as MSC scalp cooling system, CDK inhibitors(cyclin-dependent kinase inhibitors) and adenovirus-mediated expression of sonic hedgehog gene. But the results were not satisfactory.In recent years, it has been reported that Chinese herbs had the effectcs on decreasing the cytotoxic activity and enhancing the efficacy of anticancer agents. In additional, Chinese herbs were also used to treat patients with alopecia areata and androgenetic alopecia. But there are no clinical or experimental reports about prevention and management of CIA by using Chinese herbs. Fan and Zhu reported the Chinese herbs stimulated hair growth of mouse vibrissa follicles in vitro, which including astragalus membranaceus, ligustrum lucidum and olianolic acid. The hair-growth stimulation was related to the increase of synthesis and secretion of certain growth factors, such as VEGF and HGF, which have been proved to accelerate hair growth in vitro and in vivo. Therefore, it is interesting to investigate the effects of Chinese herbs on CIA. ObjectivesThis study was designed to investigate the effects of water soluble extracts of certain Chinese herbs (polygonum multiflorum thunb, astragalus membranaceus, ligustrum lucidum, ligusticum chuanxiong hort) on: (1) the hair growth of pig hair follicles in vitro; (2) protecting hair follicles from the damage induced by adriamycin in the models of cultured pig hair follicles and cultured dermal sheath cells.MethodsStudy one: The Chinese herbs protecting the dermal sheath cells of pig hair follicles from the damage of adriamycin in vitro.The dermal sheath cells of pig hair follicles were cultured with different concentration of adriamycin (0, 5, 10, 20mg/L) for 24h and with 10mg/L adriamycin for different time (4, 8, 12, 24, 48h). Then cell proliferation was detected by colorimetric assay of MTT. The optical density(OD) values were read on a scanning multiwell spectrophotometer (ELISA reader). After 24h culture with adriamycin apoptosis rate of dermal sheath cells was detected by flow cytometry. After that we celected 10mg/L adriamycin and 24h incubation as following experiment condition. In one goup, before adding adriamycin the cells were precultured with different concentration of Chinese herbs for 24h. In another group, the Chinese herbs were added with adriamycin simultaneously.Study two: Effect of Chinese herbs on hair growth of pig hair follicles in vitro.Pig hair follicles were cultured with Williams E medium (control group), Williams E medium and water soluble extracts of Chinese herbs (experimental group) respectively. Hair growth and morphological changes in hair follicle bulb were observed by microscope and the apoptosis of hair follicle cells was detected by using TUNEL (terminaldeoxynucleotidyl transferase-mediated dUTP nick end-labling) technique. Study three: Effect of Chinese herbs on adriamycin induced damage of pig hair follicle cells in vitro.Pig hair follicles were cultured in vitro with 10mg/L adriamycin or 10mg/L adriamycin plus water soluble extracts of Chine...
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