| Objective: To establish model of chronic pancreatitis and to observe its pathological and ultrastructural changes, so as to discuss mechanism of overt pancreatogenic diabetes developed by chronic pancreatitis, as well as to provide foundation for the medical research of pancreatogenic diabetes. Methods: An experimental model of chronic pancreatitis was induced by a retrograde injection of oleic acid into the rat pancreatic duct. Pathological and ultrastructural changes were investigated over a period of 6 weeks after induction of this model. Results: Histologically, the widening of acinar lumen and cellular vacuolization occurred within 24 h at the parenchyma neighboring the small ducts filled with the injected solution. Degenerative parenchyma, interstitial edema, and inflammatory cell infiltration were pronounced 1 week later. Thereafter, duct-like tubular complex formation progressed, and the exocrine tissue exhibited marked atrophy of the gland with irregular fibrosis and fat replacement after 6 weeks. Meanwhile, endocrine tissue showed overt damage. Ultrasrucrure of pancreas demonstrated progressive degenerative parenchyma, closed capillary vessel, inflammatory infiltration and fiber proliferation. Conclusion: The model of chronic pancreatitis induced by oleic acid demonstrated extensive acinar degeneration and overt damage of islet. So, it is suitable for research work of pancreatogenic diabetes.
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