Screening Of Antitumor Active Substances In Vitro From 8 Chinese Traditional Medicine And Their Mechanism Analysis

In this essay, screening of antitumor active substances in vitro and mechanism of them were investigated. The inhibitory effect of Cymbopogon distans, Lobelia chinensis, Buddleja offlcinalis, Glycyrrhiza uralensis Sanguisorba offlcinalis, Bupleurum chinense, Apium graveolen and Curuma zedoaria on the growth of two cancer cell line was tested with the MTT assay. The result indicated that ethanol soluble constituent of G. uralensis (ESCG), ethanol soluble constituent of C. zedoaria (ESGC) and ethanol soluble constituent of B. chinense (ESCB) can inhibit proliferation of SMMC-7721 and SPC-A1 cells at the concentration of 1.0-1.5mg/ml. The inhibitory rate increased in the agents concentration. The inhibitory rate of the cell line also increased with time in the range of the test. The inhibition rate of SMMC-7721 by them were : 51.6%. 48.5% and 52.9% respectively. And the inhibition rate of SPC-A1 by them were : 53.2%-. 47.9% and 58.2% respectively. At the concentration of 1.0-1.5mg/ml, the ESCB and ESCG have the best effect after 6-12 hours of treatment, and the ESCC has the best effect after 12-24 hours. The curve results suggested that the effect of ESCB, ESCG and ESCC exerting depends rather on active materials than on tumor cells.Within 72 hours obviously apoptosis such as: condensation of chromatin, apoptotic bodies in morphology can be seen in 1.5mg/ml ESCB-treating, ESCG-treating and ESCC-treating tumor cells. Blue fluorescence and red fluorescence of cells turn up by Hoechst33342 and PI staining. Also the apoptosis in tumor cells was conformed by DNA ladder formation on gel electrophoresis. The results showed that ESCG, ESCC and ESCB exhibited a marked antiproliferative effect on SMMC-7721 cells and SPC-A1 cells, and they can induce apoptosis in vitro.