1,Effect Of A Kind Of Drug-containing Serum And Five Kinds Of Traditional Herbal Mixtures In Anti-HIV Activity2,Effect Of Cu-Asp On Inhibition Of Growth And Induction Of Apoptosis In HCT-116 Human Colon Adenocarcinoma Cells

Objectives: The anti-HIV-1 activity of a kind of drug-containing serum and five kinds of traditional herbal mixtures was detected. Besides, the serologic pharmacologic method and viral inactivation assay were analysed and assessed. Methods: Anti-HIV-1 activity was detected by syncytial formation inhibition assay. KDX direct addition and rats' KDX-containing serum addition were used respectively. In addition, cytotoxicity of KZL-1,2,3, SSJK, EGCG to Human T-lymphocyte lines(C8166) was detected by MTT assay; PEG6000 solution in saline was used to concentrate viruses. Simultaneously, syncytial formation inhibition assay was used to detect the effect of HIV-1 inactivation. Results: (1) The 50% effective concentration(EC5o) of rats' KDX-containing sera (not inactivated) control group, low dose group, high dose group and KDX original reagent are l/60(v/v), l/106(v/v), l/108(v/v), 0.02mg/ml, respectively. The 50% effective concentration(EC50) of all groups of rats' KDX-containing sera (inactivated) are less than 50%, EC650 can not be calculated. (2)The 50% cytotoxic concentration(CC50) of KZL-1,2,3, SSJK and EGCG are l/34(v/v), l/41(v/v), l/1698(v/v), 1/633(v/v) , 10.27μg/ml, respectively; and the 50% HIV-inactivated concentration(INC50) are l/96(v/v),l/513(v/v), l/14(v/v), l/50(v/v), 7.29 (μg/ml, respectively. Conclusions: (l)The KDX original reagent showed obvious anti-HIV activities, EC50 is 0.02mg/ml. However, the rats' KDX-containing serum didn't showed anti-HIV activities at the dose adopted in this study. (2)The HIV-1 inactivation effect of KZL-2 was more powerful than KZL-1, and other samples all appeared to obvious cytotoxicity around the HIV-inactivated concentration range. (3)The experiment results of drug-containing serum and original reagent aren't always consistent,so we can't draw a conclusion merely according tothe result of the latter. (4)The method of Serologic pharmacologic experiment is more similar to in vivo than that of original reagent direct addition. Apparently, it is more reasonable. (5)PEG6000 solution in saline was used to precipitate viruses, and Syncytial formation inhibition assay was adopted to detect the infection activity of residual viruses,which is a simple, quick and sensitive method to evaluate potential virucidal effect of most microbicide candidates.