| Objective To investigate whether topically applied the all-trans retinoic acid (ATRA) can inhibit corneal neovascularization(CNV) in rabbits induced by alkali burn.Methods Following the central cornea of right eyes were burned by Imol/L NaOH for 30s, thirty-two Japanese white rabbits were randomly assigned to 3 study groups and a control group, each consisting of eight rabbits. The ATRA drops with different concentrations were applied topically to rabbit corneas three times daily for four weeks on each group: 15mg/L in group A, 30mg/L in group B, 60mg/L in group C, and carrier solution in group D(control). Rabbits were observed under the slit-lamp biomicroscopy and the area of CNV were calculated on days 6, 9, 12, 15, 18, 21, 25 and 28. Then the rabbits were killed on the 28th day(d), and the corneas were taken for histopathological analysis. Spearman' s analysis was used to analyze the correlation between the number of inflammatory cells and the area of CNV.Results The occurance of CNV in group A, B, C and D was (3.8?.7)d, (5.4?.5)d, (5.8?.7)d, (3.5?.8)d, respectively. The differences between both group C and D as well as group B and D were statiscally significant (P<0.05). Whereas the difference between group B and C as well as group A and D were not significant(P>0.05).At set time points, the areas of CNV in group B and C were also smaller than the group A and D(P<0.05). Histopathological analysis shows that the area of CNV had close relationship with the retrocorneal membrane and the number of inflammatory cells(P<0.05). No obvious side effect was recorded.Conclusion Topical application of ATRA has a significant antiangiogenic effect against CNV indued by alkali burn.
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