| Objective: To establish animal model of hydrocephalus in adult rat,and study the expression of vascular endothelial growth factor(VEGF) in the cerebral tissue of different sites .Methods 60 adult male Wistar rats were randomly divided into experiment group,where 48 rats were randomly divided equally into four subgroups ,i.e. group A, B, C and D, and control group. The animal models of hydrocephalus were established by injecting 0.1ml volume of 25% kaolin solution ,withthe aid of surgical microscope,into the cisterna magna in the 48 rats in the experiment group. The same volume of saline was injected into the cisterna magna in the rats of the control group..MRI was performed 1 (group A), 2(group B) , 4(group C) or 6(group D) weeks after Kaolin injection respectively to obtain T2-weighted images of brains of the rats. The rats were killed within 24 hours after MRI examination,and the brains were removed out quickly for studying the VEGF expression by the immunohistochemistry method.Results Hydrocephalus was induced in 34 rats, the ventricular system expanded along with the time passed by.The expression of VEGF is significantly increased in the cerebral tissue of rats with hydrocephalus,especially in the periventricular white matter and hippocampus.Conclusion: The technique presented here for establishing animal model of hydrocephalus in adult rat is reliable.It is particularly suitable for study of the acute and chronic pathological changes of the brain induced by hydrocephalus. The present results suggest that VEGF,on the one hand,is related to the edema that happened in the periventricular white matter,on the other hand,has neuroprotective effect to the ischemic cerebral tissue during hydrocephalus,so the VEGF probably take part in the progress of pathological injury and repair of the cerebral tissue in the rats with hydrocephalus.
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