| Objective:Invasion and metastasis are the most fundamental characters of malignant tumor and the main reasons causing patients' death. With a extremely complicated process, invasion and metastasis involve a series of interactions between tumor cells and host cells, involve over expressions of certain genes and are affected and controlled by many cell factors. The research shows that the invasion and metastasis of the tumor cells might break through the matrix around. Extracellular matrix can be classified into basement membrane and stroma tissue. Both of them are made up of basement subtance,elastic protein and collagen. During the invasion and metastasis of the tumor cells, they can play the role of protective screen. For many times, the degradation of stroma and basement membrane occurs when primary tumor transfers into invasive tumor and even when the far distance metastasis happens. In this process, urokinase-type plasminogen activator(uPA) and P-selectin play a very important role .Secreted by tumor cells, pro-uPA is inactive with single strand and may be activated into active uPA with double strands by tissue protein B in plasma. Thus, plasminogen is activated into plasmin, and most extracellular matrix are degraded. In summary, uPA participates in the process of tumor invasion and metastasis. Moreover, uPA itself may obtain the function of degradating extracellular matrix and basement membrane. And uPA may take part in the degradation of extracellular matrix elements by activating MMPs.Aparting from the primary oven is the key condition of tumor metastasis. The adhesion among tumor cells, endothelial cells and extracellular matrix is the necessary step for blood metastasis. In recent years, selectin family has been recognized as Recepter-type adhesion molecule group, including L-selectin, P-selectin and E-selectin. P-selectin may mediate the adhesion among tumor cells, platelets and endothelial cells of blood vessel so as to promote blood metastasis and diffusion of tumor cells.The research shows that uPA takes part in the pathogenesis and development of ovary carcinoma, carcinoma of stomach, carcinoma of the prostate, carcinoma of large intestine, etc. Furthermore, it has something with the invasion and metastasis of the tumors. P-selectin aberrantly expresses in the tissue of carcinoma of stomach, renal cell carcinoma , carcinoma of the lung , ovary carcinoma and concers the tumor invasion and metastasis.The relation between over expressions of P-selectin in breast carcinoma and the invasion and metastasis of breast carcinoma and the relation between uPA and P-selectin in the invasion and metastasis of breast carcinoma haven't been reported. This researchsubject investigates, through immunohistochemisty SP method, the expressions of uPA protein and P-selectin protein in fibroadenoma, carcinoma in situ and invasive ductal carcinoma tissue, discusses the role that uPA and P-selectin play in invasion and metastasis of breast carcinoma and discusses the correlation between uPA protein expressions and P-selectin protein expressions during the course of pathogenesis, development, invasion and metastasis in breast carcinoma, so as to provide theoretical basis for clinic diagnosis.Methods:Respectively investigate, by adopting immunohistochemisty SP method, the expressions of uPA protein and P-selectin protein in tissue of 25 fibroademoma cases,28 cases of carcinoma in situ and 50 cases of invasive ductal carcinoma (22 cases accompany with the lymph node metastasis).Results:1 .The rates of positive expressions of uPA protein in the tissue of fibroadenoma, carcinoma in situ and invasive ductal cancer are respectively 20.0%(5/25), 53.6%(15/28) and 88.0%(44/50). The difference of pairwise comparisons out of the three groups have statistical significance (P < 0.05) .2.The rates of positive expressions of uPA protein in the tissue of non-invasion and invasion are respectively 53. 6%( 15/28) and 88.0%(44/50). The difference in the two groups has statistical significance (P < 0.05) .3. The ra... |
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