| Liver transplantation (LTx) has become the most effective treatment for the end-stage liver diseases. However, the shortage of donor livers restricts the development of clinical liver transplantation. Brain-dead donor (BDD) is the effective way to solve the problem. Brain death is the loss of whole cerebral functions, which means death.However, some clinical experiments conclude that the organs from living donors consistently demonstrate superior results to those from cadaver sources over both the short- and long-term. It means that the organs from the brain-dead donors have been injuried before procurement.Now, the mechanism of the BDD liver injury is not clear. So, the experimental studies, which focus on the mechanism of the BDD liver injury and the prevention of it, have of great significance to the development of the liver transplantation.lt has been found that N-acetylcysteine(NAC) has the function of cleaning the free radical , inhibiting the activation of NF- K B and deminishing the levels of the inflammatory mediators. NAC has been widely used into the organ preservation and the prevention of the organ injury of ischemic reperfution. Whether NAC has the protection on the hepatic injury of brain-dead rats remains unknown.ObjectiveOur research is mainly aimed to: (1). demonstrate the functional and morphological change of the hepatic in the state of brain death and the mechanism of it. (2). investigate whether N-acetylcysteine(NAC) have the protection on the liver of the brain-dead rats and the possible mechanism.MethodsSixty Wistar rats were randomly allocated into 3 groups, 20 rats in each group: control group (group C), brain-dead group (group B) and NAC protection group (group N). Brain-dead model was established in group B and N. There was no inflation of Fogarty balloon in group C, the other operation was same with the group B. NAC at 150mg/kg was injected via the vein of the tail in group N immediately after the establishment of the brain-death model. 1 0 rats were randomly selected for acquiring specimen at 2 hour hi each group, the other 10 rats were used for acquiring the specimen at 4 hour in each group. 3ml blood from the suprahepatic vena cava were abtained at the different time points. The serum levels of ALT and AST were detected by biochemical analyzer. The serum levels of TNF- a , HA and IL-6 were detected by radioimmunoassay. Liver tissues were abtained for HE staining and detecting the activity of NF- B by immunohistochemistry. At 4 hour of each group, the morphological changes of hepatocytes and SEC were observed under electron microscope. All data were analyzed with statistic software SPSS 10.0. Results1. MAP: There was no significant difference among groupC, B, and N before inflation of the Fogarty balloon(P>0.05); in contrast to group C , the MAP in group B and N was significantly higher during inflation of Fogarty balloon(P<0.05), and was significantly lower in the state of brain-death(P<0.05); there was no significantly difference between group B and N during inflation of Fogarty balloon and in the state of brain-death(P>0.05).2. HR: There was no significant difference among group C, B, and N before the inflation of Fogarty balloon and in the state of brain-death(P>0.05); in contrast to group C, during the inflation of Fogarty balloon, HR in group B and N was significantly higher(P<0.05), and there was no significantly difference between group BandN(P>0.05).3. ALT, AST, HA, TNF- a , IL-6 and NF- K B: At the same time points, the levels of these parameters in group B and N was significantly higher than those in group C(/*<0.05), and those in group N was significantly lower than those in group B(P<0.05); At the different time points for the same group, the levels of the parameters at 4 hour hi group B was signicantly higher than those at 2 hour hi group B(P<0.05), the levels of those at 4 hour in group N was significantly higher than those at 2 hour in group N(P<0.05), there was no significantly difference between those at 2 hour in group C and those at 4 hour in...
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