Study Of Anti-tumor Effect Of Specific Immunotherapy On H₂₂ Tumor Bearing Mice

Objective: The H22 cell line and the S180 cell line arewidely applied in the tumor mechanism research and the remedyevaluation. They can produce solid tumors in the Kunming mice.In tumor study, the Kunming mice are obtained and raised easily.The H22 and the S180 tumor bearing mice are very useful tumoranimal models. It needs further study whether specific activeimmune animal models can be set up on the Kunming mice withthe H22 cells. In this study, first, the Kunming mice werevaccinated with the H22 and the S180 cell vaccines respectively,then the immune mice were attacked with the H22 cells. Furtherresearch was carried out to observe the specific activeanti-tumor effect of the H22 cell vaccine on H22 tumor bearingmice and to set up specific active immune animal models withthe H22 cell line. Immune RNAs can transfer specific immune information.Now immune RNAs are applied in clinic, including the therapyof the liver cancer, the therapy of the lung cancer, the therapy ofthe galactophore cancer, and so on. The immune RNAs areobtained from the animals immuned by various tumor tissues,and there are some debates about their therapeutic effects. In thebasal theory, the similar antigen immunity can break the 5英 文 摘 要immune tolerance of the homogeneity antigen and bring aboutthe anti-tumor effect, some problems on individual therapy mayalso be produced. In this study, the H22 tumor bearing mice weretreated by the immune RNAs from lymphatic tissues of theanimals immuned by the H22 or the S180 cells. Further researchwas carried out to investigate the volumes of the tumors, theactivity of lymphocyte proliferation, the activity of natural killercells and the phagocytosis activity of macrophages. Theresearch also compared the anti-tumor effect of immune RNAsproduced by the homogeneity tumor cell line with that producedby the different tumor cell line in the level of the experimentalanimals and the cells, and provided the suggestion for theclinical use. Methods Section one: The KM mice, whose weights were 18±1g,were randomly divided into three groups(ten mice in everygroup): the NS group(with 0.1ml sterile natural salt beinginjected into the back), the S180 vaccine group(with 5×105/0.1ml S180 vaccine being injected into the back), and the H22vaccine group(with 5×105/0.1ml H22 vaccine being injectedinto the back). The mice were injected in every seven days, andthree times in all. After three times each mouse was vaccinatedin the armpit by 2×106/0.2mlwith the H22 tumor cells. On the4th day after the H22 tumor cells were injected, the volumes oftumors were measured (V=ab2/2) and the growing curves oftumors were recorded. Eleven days later, the weights of tumors 6英 文 摘 要were weighed, the inhibition rate was counted and the activity ofCTL was tested. Section two: SD rats were immuned by the H22 and the S180cell vaccines respectively. And the two immune RNAs wereabstracted from lymphatic tissues. The RNA of unimmune SDrats was used as the control. Then the activity of three RNAswas tested by LAI-MTT. The KM mice, whose weights were 18±1g, were randomly divided into 4 groups: the NS group, theH22iRNA group, the S180iRNA group, and the unimmune RNAgroup (ten mice in every group). In the three days before themice were vaccinated, the immune RNAs and the unimmuneRNA were given with 2.0mg one mouse. Then the H22 cellswere injected in armpit with 2×106/0.2ml one mouse. At last,the RNAs were given every two days, and eight times in all. Onthe 11th day after the H22tumor cells were injected, the volumesof tumors and the inhibition rate were observed. The activity oflymphocyte proliferation, the target-cell-killing activity of NKin spleen cells and the phagocytosis activity of macrophageswere tested by MTT colorimetry. Next, the KM mice, whose weights were 18±1g, wererandomly divided into 3 gro...