The Role Of The Degradation Products Of Erythrocyte In The Brain Edema Formation Around Parenchymatous Hematoma

Objective: To investgate the role and mechanisms of the degradation products of erythrocyte in facilitating the formation of brain edema as to provide theory basis for the prevention and cure of brain edema.Methods: 1. Establishment of a model of parenchymatous hematoma: normal saline containing bilirubin or hemin, the degradation products of erythrocyte, were infused into the right frontal lobe of SD rats, and normal saline only was infused into the left frontal lobe symmetry as control. The animals were beheaded after 1, 3, 5, 7 days respectively, and the local brain tissue around the injection points of both frontal lobes were got to weigh its wet weight and dry weight. 2. At every time window, the local brain tissue around the parenchymatous hematoma were got to detect its expression of vascular endothelial growth facor by the method of immuno-histochemistry. 3. The ultrastructures of the local brain tissue around the parenchymatous hematoma were examined by electron microscope.Results: 1. At every time window, in both bilirubin and hemin group, the water content of the brain tissue specimen of experimental side is much than that of its contrast side. In both experimental group, the water content is much at the 3th and 5th day than at the 1th and 7th day. 2. In both bilirubin and hemin group, the expression intensity of vascular endothelial growth factor of the brain tissue specimen changed coincidentally with its water content. 3. By electron microscope, different degree of glial cell swelling and telangiectasia on the experimental side was seen. But, on the control side, no obvious glial cell swelling or telangiectasia was seen.Conclusion: Bilirubin and hemin, the degradation products of erythrocyte, facilitate the formation of brain edema and the degree of brain edema is correlated with the expression intensity of vascular endothelial growth factor at the local brain tissue positively, and, we suspect that the action of bilirubin and hemin is correlated to the upregulation expression of vascular endothelial growth factor at the local brain tissue.