Study On Establishment Of Experimental Autoimmune Encephalomyelitis Model In Guineapig And The Suppression Of Tripterygium Wifordii Polyglycosidium

【Objective】To eatablish the guinea-pig model of experimental autoimmune encephalomyelitis(EAE) and determine whether tripterygium wifordii polyglycosidium(TWP) can suppress both the severity and incidence of EAE by promoting the apoptosis of inflammatory infiltrating cells in the central nervous(CNS),which might provide an experimental evidence for the relevant therapeutic application【Methods】 Guinea-pigs were induced to established EAE model by being injected both guinea-pig spinal cord homogenate (GPSCH) in complete Freund's adjuvant (CFA) in four locations of the back of the guinea-pig neck and bordetella pertussis vaccine (BPV) in one footpad. The female guinea-pigs were randomly divided into control group, EAE group and TWP group. The clinical and histoneurological manifestation of the disease was followed in each of the groups. The inflammatory infiltrating cells in the CNS were counted under the microscope and the apoptosis of them was detected by terminal deoxynucleotidyl transferate mediated dUTP nick end labeling(TUNEL).【Results】1.The clinical symptoms of EAE occurred about 16 days after immunization was administered.The peak of the clinical symptoms and a distinct histoneurological change took place 3 days after the symptoms appeared. The absolute recovery necessitated a space of about 10 days.2.Compared with the EAE group ,the TWP group had lower incidence with delayed onset,lower clinical appearance , lower severity of histoneurological change and inflammatory infiltration.3.Compared with the EAE group,the apoptotic rate of inflammatory infiltrating cells in the TWP group was significantly increased.【Conclusion】The method of establishing the EAE with both CFA-GPSCH and BPV is simple ,economical and reliable.The immunosuppression of TWP has confirmed in this model,and the mechanism might be associated with its inhibition of the infiltrating and its contribution to the increased apoptotic rate of inflammatory cells in the CNS, which suggest the promising perspective of TWP in the clinical application.