Experimental Study On Effects Of Recombinant Human Growth Hormone On Serum Protein In Nephrotic Rats

Objective To investigate the effects of recombinant human growth hormone (rhGH) on serum total protein, albumin and insulin-like growth factor-1 (IGF-1) in nephrotic rats.Methods Thirty male Spraque-Dawley (SD) rats were randomly divided into control (6), doxorubincin-induced nephrotic (12) and rhGH-treated nephrotic groups (12). IGF-1 concentration in serum was measured with RIA. Protein concentration in urine was measured with ELASE. Urinary protein excreted in one day was measured with ponceau S. Total protein and albumin level in serum were measured with automatic biochemistry appliance.Results 1 Serum protein level was decreased in nephrotic rats compared with control rats. 2 IGF-1 concentration in serum was decreased in nephrotic rats compared with control rats. 3 At the end of the fourth week, IGF-1 and albumin level in serum were increased in rhGH-treated nephrotic groups. 4 There was no significant difference between rhGH-treated nephrotic groups and nephrotic groups at the same time.Conclusion The lower level of serum protein might be due to the increased loss of urinary protein and disorder of GH/IGF-1 axis in nephrotic rats. rhGH could improve the level of serum protein and IGF-1 without increasing the excretory of urinary protein in nephrotic rats.