| Malignant neoplasm is one of the most serious diseases which damage human's health. It has an important role in enhancing chemotherapeutic effect to search novel anti-cancer agents with low toxicity and high efficacy to improve the sensitivity of chemotherapy. Curcumin is a kind of plant phenol, which is extracted from turmeric. Previously reported studies suggested that curcumin had many kinds of medicinal activity, such as anti-inflammation, antiviral, dropping blood lipid, anti-atheroscherosis, anti-tumor and so on. Many reports of latest years have suggested that curcumin is an effective antimutagent and anti-cancer agent, which has been ranked as chemoprophylatic agent of the third generation. Many reports have placed stress on tumor cells with blood metastasis, there are no reports about cancer cells with lymphatic metastasis. Our study initially explores the mechanism for anti-tumor effect of curcumin on cancer cells with lymphatic metastasis. In vitro, we make two murine hepatocarcinoma strains (HCa-F and HCa-P) with high and low metastatic potential as our research objects, exploring effects of cucurmin on some biological characteristics of them. As we all known, the metastasis by lymphatic tract is the second pathway in hepatocarcinoma. So our study is not only of theoretical significance, but also of potentially applicable usage. At the same time, this study can provide reliable theoretical proof in exploring natural anti-tumor agents with cheaper price, lower toxicity and higher efficacy.Methods: MTT assay is used to detect the uncytotoxicity doses of curcumin and count 50% inhibitory concentration(IC50).Two kinds of cells are treated with the uncytotoxicity doses of curcumin and the morphological changes of the cells are observed respectively. The influence of the curcumin on cell cycle are shown by using Flow Cytometry(FCM). HCa-F cells are inoculated to 615 mice via oral administration or intraperitoneal injection and the effects of curcumin on the formation of tumor and the metastasis by the pathway of lymphatic tract are investigated. Results: 1 Curcumin at the concentration of15.625umol/l-250umol/l can obviously inhibit the cells of two strains growing in a dose-dependent manner(P<0.05) after HCa-F and HCa-P cells have been protreated with curcumin for 48 hours. The IC50 of HCa-F is 51.48umol/l, while the IC50 of HCa-P is 86.48umol/l, the latter is higher than the former.2 When curcumin is 15umol/l, the livability of HCa-F and HCa-P are 92.77% and 93.96% respectively, which are greater than 90%, and thus 15umol/l is the uncytotoxicity.3 In contrast to the control group, at the concentration of 15umol/l of curcumin can make HCa-F and HCa-P decreasing, but there is a mild morphologic change.4 Compared with the control group, Curcumin of 15 umol/l can increase the colony doubling time of the two kinds of cells. From the growth curve, we can see curcumin of 15umol/l can inhibit the growth of the two kinds of cells obviously.5 Cell cycle of HCa-F is blocked in S phase, but that of HCa-P is blocked in S and G2/M phases after the two kinds of cells were treated with curcumin of 15umol/l.6 Curcumin at the dose of 50mg/kg body through abdominal cavity for 7 days can inhibit the formation of ascites after 615mice was inoculated HCa-F through abdominal cavity.7 After 615 mice were given HCa-F through hypodermic inoculation, curcumin at 150mg/kg body via oral administration can make the life span of 615 mice prolonging to 45.06%.8 The lymphatic metastasis decrease from 70% to 40%, after the mice have been given curucmin at 50mg/kg for 15d via abdominal cavity injection. Conclusion:1 Curcumin can inhibit the proliferation of HCa-F and HCa-P in dose-dependent manner and the IC50 of HCa-F is lower than that of HCa-P. Curcumin at uncytotoxicity concentration can make doubling time of HCa-F and HCa-P prolonging and have cell cycle distributed, but HCa-P repairs more quickly, a few cells of which have passed through G2/M check point. So HCa-F is more sensitive t... |
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