Plasmid Construction And Idetification Of Human Vascular Endothelial Growth Factor Receptor (VEGF-R3) Extracellular Domain 1-3Loop CDNA

Objective: To insert VEGF-R3(FIT-4) gene fragment into cloning vector and identify itMethods: By T-A cloning, the VEGF-R3 gene fragment amplified by RT-PCR was cloned on the PGEM-T Easy plasmid vector and transfected into E.coli JM109. the recombinant clones were screened by "white-blue plaque selection" and tested by the methods of single restrictional enzymeResults:The VEGF-R3 gene fragment amplified by RT-PCR showed a smear after the electrophoresis on agarose Gel and obtained white clones by "white-blue plaque selection". The electrophoresis result of the recombinant clones tested by the single restrictional enzyme was accordant with expectant result.Conclusion: The recombinant clones through the methods T-A may be used in the further gene expression and gene therapy studies...