Effects Of Dexamethasone On VEGF-C And VEGFR-3 In Primary Culture In Vitro Of Laryngeal Squamous Carcinoma Tissue

Objective: The expression of VEGF-C,VEGFR-3 was studied by defectiving in primary culture in vitro of laryngeal squamous carcinoma tissue in order to explore the node metastasis of laryngeal in clinic by dexamethasone(DEX).Methods: Applying the technique of primary tissue culture in vitro, twenty specimens, which were identified pathologically as laryngeal squamous cell carcinoma, were cultured. After succeeded culture of twelve specimens, the cells were divided in two groups. One continues to culture, another give 10-7 mol/L of DEX. An immunohistochemical were analysis with antibodies to VEGF-C, VEGFR-3 were performed, VEGF-C and VEGFR-3 gene express in 12 cases of cells were examined by reverse transcription polymerase chain reactios (RT-PCR)Results: 8 of 12 specimen were VEGF-C positive and 7 of 12 specimens had VEGFR-3 overexpression,they were all remarkably higher than that in the control groups. The gene expression of VEGF-C and VEGFR-3 is different between two groups. Expression of VEGF-C VEGFR-3 in laryngeal primary culture in vitro was significantly higher than in laryngeal primary culture in vitro by DEXConclusion: The expression of VEGF-C and VEGFR-3 in primary culture in vitro of laryngeal squamous carcinoma tissue by DEX were decrease .It may provide guide to apply DEX in clinic laryngeal obstruction.