The Study Of Protective Effects Of SHEN-FU Injection On Rabbit Myocardium During Ischemia-Reperfusion

Objective: To investigate the protective effects of SHEN-FU injection on myocardium during ischemia-reperfusion, and explore its possible mechanism, thereby providing rational clinical application.Methods: Using rabbit ischemia-reperfusion injury model , thirty-two healthy rabbits of both sexes were randomly divided into four groups of A, B,C and D. Group A (sham operating group): The chest was opened and left anterior descending (LAD) was exposed with no further procedures. Group B (operative control group): LAD artery was occluded and the occlusion was maintained for 45 min and released for reperfusion (120 min). Before LAD occlusion, the NS were administered intravenously at the rate of 0.5 ml/min by an infusion pump. Group C and Group D (different therapeutic groups): The procedures were the same to group B, but the different dose of SHEN-FU Injection were administered intravenously at the same speed by an infusion pump. The index of cardiac hemodynamics and the electrocardiogram of rabbits were respectively recorded with LMS-2B system and electrocardiograph. The infract size of rabbit hearts were measured by dual staining with TTC-Even's blue dye. Serum creatine phosphokinase(CPK), lactate dehydrogenase(LDH), superoxide dismutase(SOD) activities and malondialdehyde (MDA) concentrations were also measured in each group. Apoptotic myocytes were detected with transmission electron microscop and terminal deoxynucleotide transferase-mediated deoxyuridine-biofin nick end labeling (TUNEL) method. The expression of caspase-3 protein and bcl-2 protein were detected by Western blot and Immunohistochemistry analysis.Results: There was no statistically significant difference in heart rate (HR). Compared with group B, left ventricular systolic pressure (LVSP), left ventricular end diastolic pressure(LVEDP), and 眃p/dtmax were restored, but with no statistically difference duringischemic period (P>0.05). During the reperftision period, LVSP, LVEDP, 眃p/dtmax were better restored(P<0.05). Simultaneously, the occurrence of arrhythmia after perrusion was lower in group C and group D than in group B. The activity of CPK, LDH in the blood plasma and the level of MDA were lower in the group C and group D than those of group B (P<0.01) ,while the activity of SOD was higher (P<0.01) . The myocardial infarct size was also reduced in the rabbits treated with SHEN-FU Injection. The occurrence of apoptosis was significantly higher in group B than in the group A, but significantly fewer in group C. Compared with the group A, The expression of caspase-3 protein and bcl-2 protein was both significantly higher in group B (P < 0.05 ). The expression of bcl-2 protein was significantly higher (P<0.05) ,while the expression of caspase-3 protein was lower in group C (P<0.01) .Conclusion: (DSHEN-FU Injection exerted obvious protective effects on myocardium during ischemia-reperfusion. SHEN-FU Injection can accelerate the resume of myocardial function during ischemia-reperfusion, countermine arrhythmias after reperfusion, reduce the myocardial infarct size. The mechanism might be associated with that SHEN-FU Injection can antagonize oxygen free radicals, correct intracellular calcium overload, enhance myocardical function. (2) SHEN-FU Injection can inhibit the apoptosis induced by myocardical ischemia-reperfusion. The expression of caspase-3 protein dropped, while the expression of bcl-2 protein upgraded in the SHEN-FU Injection treating group (group C). The mechanism might be associated with that SHEN-FU Injection can boost the expression of bcl-2 protein and inhibit myocardical apoptosis, with the dropping expression of caspase-3 protein.