The Preparation, Biology Evaluation And Cell Culture In Vitro On The Hyper-porosity Ca₃(PO₄)₂ Bone Cells Scaffold/NM ZrO₂ Reinforced

Objective: To develop a new degradable hyper-porosity Ca3(PO4)2 bone cells scaffold/NM ZrO2 reinforced, evaluate it's biology toxicity and culture osteoplast cell in vitro.Method:.Degradable hyper-porosity Ca3(PO4)2 bone cells scaffold/NM ZrO2 reinforced was made from hydroxyapatile and namo ZrO2 grain. Leach liquor of Bone Cells Scaffold/NM ZrO2 was injected into rat tail intravenous and the toxicity response was observed after 24h, 48h and 72h . The degree of rabbit erythrocatalysis and nomadic haemoglobin were measured in order to evaluated the hemolysis degree in vitro. HOS-8603 cells' phenotype and their relative survival rate were observed after exposure to leach liquid . The combination of the cells and the scaffold was detected through scaning electron microscope. Primary osteoblast cultured from rabbit marrow stromal stem cells were identified by alkaline phosphatase staining and activity of alkaline phosphatase assay . Osteoblast were cultivated on Scaffold/NM ZrO2 to measure the growth state of cells by MTT assay.Result: Porosity of the scaffold is 75-93%. Pore diameter is 200-450um. Pore distribution is well distributed and linked up. Compressive strength is 2.0Mpa. Cytotoxicity of Bone Cells Scaffold/NM ZrO2 is 0-1 grade. There was not significant toxicity behave, body weight decrease or death in rats group being injected leach liquor. Hemolysis rate is 2.064%. HOS-8603 cells grew as well in scaffold leaching liquor as in normal team. Observed through scaning electron microscope , the cells grow well . Osteoplast comes out when marrow stem cells were cultured in induction nutrient fluid. Result of staining is "3+" The assay of activity of alkaline phosphatase proved that osteoblast had been induced successfully. Osteoblast grew well on the scaffold. Multiplication was obvious. The amount of cell increased obviously along the culture time.