The Killing Effect And Induced Apoptosis By Low-intensity Ultrasound With Hematoporphyrin Derivatives On EAT Cell In Vitro

According to the predication of WHO, malignant tumor will be the first killer to human health in the 21th Century. As far as the actual condition is concerned, any single conventional cancer treatment available (resection, chemotherapy or radiotherapy et al) can hardly be a radical therapy to the patient with malignant tumor, especially those with middle or late stage ones on admission, combined therapy has been the main therapeutic means gradually. On the foundation of photodynamic therapy(PDT), Shin-ichiro V memura, a Japanese scholar, has brought forward a new antitumor theory-sonodynamic therapy(SDT). The antitumor effect of SDT is based on the synergistic effects induced by photosensitizer which can remain preferentially in tumor tissues much longer than in normal tissues when activated with ultrasound. Many scholars have paid close attention to SDT for its theoretical significance and clinical value.A rapid development has been got on the studing of SDT since its putting forward. At present, the studies on the SDT have focused mainly on the mechanisms of killing effects on the human cancer cells in vitro and animal tumors in vivo by using different ultrasound parameters and different photosentizer. So far the studying has been still in the stage of lab and has a far way for clinic. Based on the early studying results of our laboratory, studies of home and abroad, and assuming a part of work of "The Mechanism of Tumor Cell Apotosis Induced by Ultrasound Activated Hematoporphyrin"supported by National Nature Science Foundation, I adopted the frequency of ultrasound wave 1.43MHz, the low-intensity 1W/cm2 with duration of exposure of 120s in this paper to observe the antitumor effects on Ehrlich ascites tumor (EAT) in vitro and the potential mechanisms of action inducing this cytotoxicity, in order to promote the development of SDT and put it into clinical pratice early. The main conclusions are as follow:1. Morphology observed through sample preparation for different time that hematoporphyrin can significant enhance the damage degree of EAT induced by low-intensity ultrasound and lingering damages, the sensitive killing sites of SDT are membrane system and mitochondrion is the most site to be destroyed.