Experimental Studies On Reconstrction Of Rabbits Mandibular Angle Defects By BMSCs/Degradable PHB Composite Graft

An ideal scaffold material is one of the popular studies on bone tissue engineering. Many different scaffold materials are being explored, which have their own characteristic origin and diverse properties. In this experiment, a kind of new degradable polymer PHB was studied as a scaffold in bone tissue engineering, and rabbit's mandibular defects were reconstructed by BMSCs/PHB composite graft successfully.Part oneObjective: BMSCs were isolated and cultured in vitro as the seed cells in followed bone tissue engineering research. Different proliferation characters were investigated during the primary culture, subculture and induced culture. Methods: BMSCs of rabbits cultured in vitro were induced to become osteoblasts by condition medium (10%FBS 10nmol/Ldexamethasone, 50mg/L L-vitamin C and 10nmol/LP-sodium glycerophosphate in DMEM). Osteoblasts were indentified by histochemical staining of alkaline phosphatase (ALP), and the cell configuration and growth characters both types of cells were investigated by inverted microscope and cell counting method. Results: BMSCs were differentiated to become osteoblasts, and to be stained positive by alkaline phosphatase after induced by condition medium. The obvious difference of cell growth between inducedgroup and control couldn't be found by the growth curve obtained by cell counting method. Conclusion: The cultured BMSCs in vitro could be used as the seed cells for bone tissue engineering research.Part twoObjective: To study the biocompatibility of PHB and the effects of bFGF and BMP-2 on BMSCs' proliferation. Methods: ?The BMSCs were co-cultured with PHB verse BMSCs cultured alone. The cell proliferation and the adhering characters were observed by inverted microscope and scanning electronic microscope; (2)bFGF or BMP-2 was added into 96 pore culture plate as the different group. Cell proliferation was investigated with MTT assay on Id, 3d, 5d, 7d. Results: (T)BMSCs and cell juncture can be found on the material surface during the co-culture with PHB by scanning electronic microscope. (2)bFGF, BMP-2could promote the proliferation of BMSCs. The effect of bFGF is more significant than BMP-2. Conclusion: PHB have fine biocompatibility with BMSCs. Both bFGF and BMP-2 can promote the proliferation of BMSCs.Part three:Objective: To learn the capacity of different materials to repair rabbits' mandibular angle defects and to investigate the biocompatibility of PHB as bone reconstruction scaffold. Methods: The size of 0.8cm XL Ocm on mandibular angle defects in rabbits were made, then repaired with artificial bone composite respectively according planned groups. The results were evaluated by general observation, radiological examination, histological method and scanning electronic microscope. Result: The defects...