The Study Of The Effects Of Sodium Selenite And All-trans Retinoic Acid In Lung Cancer Cell Strain A549

PrefaceRetinoic acid including vitamin A in nature and ramification of artifical synthetic is a combines of multitude species, the Retinoic substance which sunthe-size in bodys from it obviously has the effects on inhibition of proliferation and induction of differentiation. Sodium selenite, the representation of selenium combines, is the necessitous trace element of the clays vital activities. Investigations have approved that selenium is the trenchancy inhibitor of galactophore cancer, liver cancer, skin cancer, colon cancer and stomach cancer, some investigations in outside of body indicated that the induction of differentiation in a number of tumour cell lines by Retinoic acid and Sodium selenite had taken out adding effects, namely, the rate of induction of differentiation and inhibition of proliferation on malignant tumors by the associated application of two different sorts medicaments is approximately double towards by the every one.Retinoic acid and Sodium selenite could decrease the mutation rate of carcinogenic agents, protect germ plasm, inhibit mitose and proliferation optional, induce cancer cells differentiation and reversion, so they are perfectly preventa-tive and resistive agents against tumors. The investigation of Sodium selenite and All - trans Retinoic acid on induction of differentiation has become the hotspot in investigation on prevention against and remedy for tumors. But the investigation on entity tumors is still tiny, specially on lung cancers. To increasing the curative effects of druggery, decreasing drug - resistance, it is necessary get more advance investigation on adding effects of Sodium selenite and All - trans Retinoic acid. The distinguish and affiliation between Sodium selenite , All - transRetinoic acid and chemotherapeutic remedys, and the mechanism of Sodium sel-enite and All - trans Retinoic acid is awaiting to more lucubrate. The effects of All - trans Retinoic acid on mitochondria and the feasibility of mitochondria used as guideling for entity tumors deserve to father more exploring.ObjectiveTo investigate the mechanism of different doses of Sodium selenite and All — trans Retinoic acid on inhibition of proliferation and changes of cell cycle and mitosis index in lung cancer cell line A549. To investigate the dose -effect and time - effect relationship of Sodium selenite and All - trans Retinoic acid on changes of optical density , cell cycle and mitosis index in lung cancer cell line A549.MethodsThe lung cancer cells A549 were cultured and treated by Sodium selenite, All - trans Retinoic acid, Sodium selenite combined All - trans Retinoic acid at the concentration of 2. 5 (xmol/L, 5. 0-μmol/L, 10.0μmol/L and 20.0μmol/L, respectively, and at different time interval . The MTT colorimetric assay was used to analyze the optical density. Flow cytometry (FCM) was used to detect the percentage of cells at G₀/G₁phase, S phase and G₂ + M phase . Taked cells fixated and dyed , counted mitotic cells with microscope, then worked out mitosis index. A total of five groups were designed, the blank control, 0.095% volume ethyl alcohol control , Sodium selenite groups, All - trans Retinoic acid groups, Sodium selenite combined All - trans Retinoic acid groups.ResultsThe optical density of Sodium selenite, All - trans Retinoic acid groups of 2.5μmo/L - 20. 0μmol/L and combination of the two drugs at the same doses as above groups that had treated over 24 hours - 96hours was significantly high-...