Study On The Biological Effect Of β-TCP Ceramics

β-TCP ceramics have been widely used into clinical as the bone-repair and bone-substitute material. However, the bioactivity of β-TCP ceramics is still not explained clearly, especially bone-related gene expression is reported lesser. Therefore this study is aim to provide the theoretical foundation for the clinic use of the β-TCP ceramics by exploring its effect on the osteoblast and protein using the cell biological and molecular biologic methods.First, osteoblast was cultured using improved method of the enzymic digestion and tissue piece. The cells were identified from the cell morphology, ALP activity, and calcium nodule. The result showed that the cell had the morphological and biological feature of osteoblast and this provided the active cell basis for the future experiment.Second, the co-culture experiment showed the β-TCP ceramics could promote osteoblast from G₀/G₁ phase into S phase, accelerate the cell into the DNA synthesis phase to promote the DNA synthesis and cell proliferation; RT-PCR showed the β-TCP ceramics could increase osteocalcin (OCN) gene expression, this made the osteoblast into a new mature stage, improved the activity and promoted the differentiation of osteoblast. The mechanism maybe is that the degraded product of P-TCP ceramics can provide substantial basis for the osteoblast to grow, proliferate, secret and different into osteocyte.Last, the adsorption ability of the P-TCP ceramics to the protein was studied. The result of XPS, SDS-PAGE showed the p-TCP ceramics has a strong affinity and the absorption layer is higher than 10nm. In XPS, the chemical displacement of the Ca2p binding energy illustrate there are chemical absorption at the β-TCP ceramics surface; In FT-IR, the half -peak width of the amide I bands narrowed down, the force improved and the site changed. The amide I bands was studied further by the de-convolution and second derivative technique. The fitting result showed the β-TCPceramics could increase the α-helix structure and decrease the β-fold, corner and random structure of BSA and transform the random structure into the order structure. This maybe caused by the chemical bind between the β-TCP ceramics and BSA then changed the secondary structure. Those changes are benefited for the biological mineralization of the material in vivo.