The Effect Of Propofol,Midazolam On Fos,Jun Protein Express In Rat Myocardial Ischemia And Reperfusion Injury

Objective: Myocardial ischemia and reperfusion injury molecular mechanism was not sure ,many researchs confirmed myocardial ischemia and reperfusion injury result the expression c-fos , c-jun gene,caused Fos , Jun protein increase. This research,we used myocardial ischemia reperfusion injury animal models, advanced test about propofol and midazolam for the effect of Fos, Jun protein express in rats myocardial ischemia and reperfusion injury. We tried to find propofol , midazolam the relationship of protective myocardial ischemia in gene expression lever, in order to give the direction clinical work in cardical surgery and heart disease patient how to use the anesthesia drugs. Materials and methods: Sixty adult SD rats, fourty-eight adult SD rats , anterior interventricular branch were ligated in 15 minutes,then the ligation were removed.Twelve adult SD rats did sham operation. (l)Propofol group: Twenty-four rats were randomly divided into 12 rats A: experimental group(used propofol introvenous,the dose 2mg/kg/h),12rats B: control group (usedsodium chloride introvenous) , each group divided 6 time phase got myocardial tissue, I :ischemia 15 minutes, II :reperfusion 15 minutes,III: reperfusion 30 minutes, IV :reperfusion 60 minutes, V :reperfusion 120 minutes, VI : reperfusion 240 minutes.(2)Midazolam group: Twenty-four rats were randomly divided into 12 rats C: experimental group(used midazolam introvenous,the dose 0.2mg/kg/h) .12 rats D: control group( used sodium chloride introvenous),the time phase were the same as the propofol group.(3) sham group: 12 rats, anterior interventricular branch were not liganded(used sodium chloride introvenous), the time phase were the same as the propofol group.Immunohistochemistry technique was used to analyse the amount change of the Fos, Jun protein expression. Results: Propofol group: (1)A group Fos protein expression gray change, compare each group IV time phase was significant higher than other group(P<0.01 );B groupIIL, IV , Vtime phase were significant higher than I , II (P<0.01), IV time phase was significant higher than III(P<0.01). Between two group,A group II, III, IV, Vtime phase were lower than B group Fos protein expression (P<0.01) . (2) A group Jun protein expression gray change, compare each groupIIL IV time phase were significant higher than I , II , V, VI (P < 0.01); B groupIII, IV, V time phase were significant higher than I , II(P0.01), IV time phase was significant higher than V, VI (P<0.01) . Between two group ,Agroup IIL IV, Vtime phase were lower than B group Jun protein expression (P < 0.01 ).Midazolam group: (1)C group Fos protein expression gray change, compare each groupIII, IV , V time phase were significant higher than I . II > VI (P < 0.05), IV was significant higher EH (P<0.05);D groupHL IV , V time phase were significant higher than I , VI(P<0.01), IV , V time phase were significant higher than I, II , VI(P<0.01). Between two group ,C group IV % V time phase were lower than D group Fos protein expression (P < 0.01) . (2)C group Jun protein expression gray change, compare each groupIV time phase was significant higher than I , II, III, VI (P < 0.01); D group IV, V were significant higher than I , II, III, IV(P<0.01), IVtime phase was significant higher than V(P<0.01). Between two group, C group IV , V time phase were lower than D group Jun protein expression (P < 0.01).Sham group E group : there have no Fos, Jun protein expression.Conclusion: This experinment demonstrated myocardical ischemia reperfusion injury cause Fos, Jun protein express, propofol and midazolam decreased oxygen freedom radical production in myocardical ischemia reperfusion injury, protected myocardical function.Both drugs caused Fos, Jun protein express decrease in myocardical ischemia reperfusion injury.These caused c-fosN c-jun gene expression decrease,inhibited the myocardical apoptosis.these...