Effects Of EGB761 On Primary Cultures Of Embryonic Rat Mesencephalic Cells

Parkinson's disease (PD) is characterized by a progressive and massive degeneration of mid-brain dopaminergic neurons, primarily from the substantia nigra pars compacta(SNpc). It is a neurodegenerative progressive disorder prevalent in the population above 45 years of age. The clinical symptoms are caused by the loss of dopaminergic neurons that exceed 50% of all and the consequent dysfunction of the nigrostriatal pathway. The disease is detected as a mild resting tremor of one or more limbs,muscular rigidity, postural abnormalities, and bradykinesia-akinesia. The mechanism and pathogeny by which dopaminergic neurons degenerate in PD is still unknown. Yet,increasing evidence suggests that free radical-mediated oxidative stress may participate in the mechanism of nerve cell death. Indeed, decreased activity of complex I in the mitochondrial respiratory chain and the toxicity of excitatory amino acid (EAA) ,and a high level of lipid peroxidation have been reported in the SN of Parkinsonian patients. These cellular and molecular alterations ultimately lead to apoptosis. The crude prevalence of PD was estimated to be 0.103 per 100 inhabitants of whole human being. The prevalence of PD above 55 was 1.02 per100 inhabitants in our country. L-dopa is still the most effective and commonly prescribed treatment for PD. However, long-term treatment with L-dopa is associated with numerous, well-known complications that are extremely discomforting for the patient. These include "on-off" fluctuations, freezing episodes, "wearing off', above all, abnormal, uncontrollable movements, known as dyskinesias. We had found that the ginkgo biloba extract (EGB) had effection on the parkinson's diseases patients. Although the cellular and molecular mechanisms underlying the action of EGB on cellular protection are not fully understood, the antioxidant effect might be a possible mechanism for EGB-mediated neuroprotection. we investigated the possible mechanism of EGB in the prevent and therapy of Parkinson's diseases. First ,we observe the effects of EGB761 on primary cultures of embryonic rat mesencephalic cells. Next we investigate the protective effects of EGB761 on MPP+-injured embryonic rat mesencephalic cells in vitro.Experiment 1Objective: To investigate the effects of the EGB761 on the primarily cultured neuronal cells from embryonic rat mesencephalon. Methods: Primarily cultured neurons were prepared from embryonic rat mesencephalon after treated with EGB761 and NGF in different deliveries in vitro. Mesencephalic cells were observed and their viability was assessed by MTT assay and the number of tyrosine hydroxylase (TH) positive cells was counted with immunocytochemical staining. Eventually, the number of positive cells and their neurites of outgrowth were compared between different groups . Results: The number of TH-positive cells were significantly higher in EGB761 or positive control groups(neural growth factor groups, NGF groups) than in the control groups(P<0.01) ,and the viability...