Effect Of Hujin Granules On Hepatic Fibrosis Induced By Pig Serum In Rats And Its Possible Mechanism

Heptic fibrosis is a pathologic process that the fiber tissue intra-hepatic increases abnormally When the liver cells necrosises or Inflammation stimulates. Its main future is the excessive accumulation of the extracellular matrix (ECM) and which is due to an imbanlance between synthesis and degradation of components. The reason for it include viral hepatitis (HBV,HCV),bile duct obstruction , metabolic diseases , alcohol abuse and chronic toxin enposure etc. In the former experimental and the clinical research , it has been proved this disease a prelude of various chronic livers disease turnning to be hepatocirrhosis or liver cancer. Therefore, both domestic and overseas scholars have paid a great deal attention to the prevention of it . Up to now, though the reversibility of this disease has got the proof, the clinical application lacks the specially good effect medicine still. The popular drugs such as colchicine interferon etc can inhibit the hepatic fibrogenesis. But because of their side effect and the instability, they are confined to use widely. For this reason, our Tradition Chinese Medicine drew on more and more of vision due to its good exhibition in this leve.Heptic fibrosis turns to belong to the hypochondrium pain or abdomin- al mass according to TCM theory. During the pathogenesis of hepatic fibrosis, stagnation of qi , blood stasis and the disturbance of functions of liver exist in the whole process of it. Above all, blood stasis is the fundamental pathomechanism. Therefore,the treatment of hepatic fibrosis Includes promoting qi flow, soothing liver .strengthening spleen,soften-ing , removing food retention and harmonizing all organs, especially activ-Ating blood to resolve stasis and nourishing qi to resolve stagnation.Hujin pills is created by professor Huang Zhao-Sheng through his clinician experience. This compound consist of six herbs. In this compound, Rhizoma Polyg-onicuspidati and Radix Curcumae could promoting blood to resolve stagnation and promoting qi folw to stop pain as principal drugs;Fructus Crataegi and Radix Notoginserg could promoting blood to resolve Stagnation; Rhizoma Alis-atis could promoting urination to removing dampn- ess. Both of them were be minister drugs; Ganoderma could replenishing qi and reinforcing deficiency as adjuvant drug. Our thesis was based on the optimizing the arts and crafts and improving on the form of prepared drugs, later the Hujin Granules were gotten. This aim of study was to investigate the possible effects and mechanism of the compound on experience hepatic fibosis.In this experience , SD rats were randomly allocated into six groupsta normal control group, a modle control group, colchicines group and the low (0. 6g/kg), media(1. 2g/kg), high(2. 4g/kg) group of Hujin Granu- les. The latter 5 groups were administered with pig serum by subcutaneous injection biweekly (0. 5ml/sample) for a period of 12 weeks. On 12 weekends, put animals to death , fetches some anticoagulatory bloods ,separate serum and animal' s liver to measure index of the hemodynamics , liver function, serology and cell factor secrete competence , observes pathology morphology of liver and organizes the ultra micro-structure change under mere mirror and electric mirror. In addition , adopt RT-PCR method to observe TIMP gene expression level also.Serum measure result indicate different dosage group of Hujin Granules have obvious inhibition to ALT, AST, thus achieve the goal of improving liver function in various degree. At the same time the high dosage group demonstrates the remarkable difference compare with the group of the colchicum. In addition, different dosage group of Hujin Granules can also obviously prevent or lighten the liver fibre . It is shown as the fibres index of the model animal's serum, suchas HA, LN, PC III, IV- C , dropped significantly.The result of pathology morphology and ultra micro-structure shows , traditional Chinese medicine treat group liver blood hole and central vein structure immovable basically, the blood circulation is normal, the fibrous tissue is rare in hyperplasia. The cell membrane of liver of the greater part keeps the ideal normality , liver cell necrosising obviously reduces, mitochondria swelling degree and glycogenic particle lose situation lighten notably, kuffer cell less than Western medicine group also; Colchicum have certain treatment function too , but intrahepatic collagen fibre content exceed traditional Chinese medicine treat group obviously and which interweave complicatedly in liver.Cell factor secrete competence measure result indicates, compare with the group of model, different dosage group of Hujin Granules can make TNF-a, IL1β obviously reduced .Moreover, the influence of traditional Chinese medicine treaty on hemodynamics embody in in many aspects .on the One hand, it is able to reduce the hematocrit and plasma viscidity notably . On the other hand , it can reduce the the index of red blood corpuscles togather, thus demonstrate the whole blood viscidity and whole blood reduce viscidity to drop.From the molecular biology aspect, we can see too, the normal liver has TIMP to duplicate, but model group expression level of it rise notably, the group of Hujin Granules can reduce expression of TIMP obviously and there is a remarkable meaning compared with the former.Synthesizing the former result, we think that the Hujin Granules have good effect on protectting the liver function, lowing enzymes, suppressing liver fibre hyperplasia. Its mechanism might on one hand with reduce cell's factor secrete competence, improve the mouse' s rheology performance of blood, promotes and resume the microcirceulation of liver district, lighten the liver cell to damage , thus weaken the function of activating on HSC . on the other hand, have something to do with resuming MMP activation through suppressing...