Studies On The Pharmacologic Substance Basis Of Tongmai Oral Liquid

Objective:Tongmai oral liquid possesses a good curative effect for chronic nephritis in clinic, which had been proved by Guangdong Province Hospital of TCM for a long time. This paper provides scientific bases for compositive mechanism, clinic application and treatment mechanism of Tongmai oral liquid through such research work respectively: The quality control of materials for Tongmai oral liquid, extracting and purification technology for main chemical parts, screen out pharmacological effect of different chemical parts, effective chemistry part's fingerprint research, separation and identification of main components of Tongmai oral liquid.Methods:On the basis of medical literature, the quality control method for medicinal materials of Tongmai oral liquid was established by using Tin-layer chromatography method to differentiate and spectrophotometry to mensurate the content of polysaccharides and total saponins; single factor investigative method and orthogonal experimental design were used to screen out the extracting process of Tongmai oral liquid. The macroporous resin D101 was used to gather and purify total saponins, the pharmacodynamic effect of different chemical parts of Tongmai oral liquid was investigated with blood rheology index and immunization parameters; the fingerprint of total saponins which had been proved effective was studied preliminarily by using Tin-layer chromatography method and HPLC method; The main chemical components ofTongmai oral liquid were separated and purified by using Column chromatographic technique, Crystallization method etc, Combined with IR, MS,¹HNMR, ¹³CNMR, HPLC method to identify these components.Results:1 The results of quality control method study for medicinal materials in Tongmai oral liquid:In Tin-layer chromatography of Astragalus menbranaceus (Fisch.)Bge. , Astragaloside is yellow blot, R_f value is 0. 35, the Tin-layer chromatography action does well. In the Tin-layer chromatography of Panax notoginseng (Burk.) F. H. Chen, the blots of Ginesenoside Rg, and Ginsenoside Rbt are amaranthine blots, R_f value is 0.15 and 0.37 respectively, the Tin-layer chromatography action does well too. Measuring several batch samples, we found the contents of Astragalus menbranaceus (Fisch. )Bge. polysaccharides are between 5. 56%⁷.18% ; the contents of Panax notoginseng (Burk.) F. H. Chen saponins are between 6. 96%⁸. 10%.2 The results of extracting and purifying process study:The condition of extracting process for Tongmai oral liquid was optimize by single factor investigative method and orthogonal experimental design, using the yield and content of polysaccharides as parameters. The optimum extractive process is: soaked for 12 h, extracted twice with 8 times of water,3 h per time. The yield and content of saponins were 2. 18%². 54 and 68. 51⁷7. 23% respectively by the method of macroporous resin D101 gathering and purifying.3 The results of pharmacodynamic study on different chemical parts of Tongmai oral liquid:The results on immunity function of mice showed that the immunity function of group TM1, group TM2, group ZG and group DT were improved in different degree, (P<0.05-0. 01), while the group TM3's mice hardly affected on the immunity function( P>0. 05).The results on the blood viscosity of rat showed that group TM1, group TM2 and group ZG can decrease the whole blood viscosity and plasma viscosity in different degree (P<0.05-0. 01), while group DT and group TM3's rats haveno changes on viscosity