| Based on the investigation of literature about Leonurus japonicus, this article made a study of Leonurus japonicus in three parts: quality standard, extraction process and dosage form change.In the first part, to improve the TLC method to identifiy stachydrine, different developers and different chromogenic agents were used, the developer consisted of EtOAc-BuOH-HCl (3:2:1) had best resolution, and the chromogenic agent composed of KBiI4-KI·I2(1:1) was best sensitive to stachydrine. Compared with old method, the new one takes only 30min to complete the process. To determinate the stachydrine in Leonurus japonicus by HPLC, the column used was Waters-NH4(250mm × 4.6mm, 5μm), the mobile phase used was acetonitrile-water (80:20) which was pumped at the rate of 1.0mL/min, the detection wavelength was set at 202nm, the column temperature was controlled at 25 ℃ .Under this conditions, the linear range of stachydrine was 0.5-10μg, r=0.9999. The method recovery was 98.5% and the RSD was 1.95%. Comparison of the analysis results with HPLC and ammonium reineckate spectrophotometry was made, and it was found that the former were more precise than the later. The newly established method was used to determinate the stachydrine in different preparations of Herba Leonuri.The result showed that content of stachydrine in most products is low, which can't reach the Chinese pharmacopoeia standard. Furthermore, there were significant differences among Herba Leonuri preparations from different pharmaceutical factories, place of origin and batches. So it is necessary to set the assay tolerance of stachydine in products with Herba Leonur.In the second part, to study the undefined conditions of Herba Leonur extraction process which was recorded in Chinese pharmacopoeia, the experiments were carried out with parallel tests, the process conditions were ascertained by determining the solid content and transferring rate of total alkaloid. To look for new extraction process for Herba Leonur,... |
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