The Effects Of Captopril And Honghua Extractives On The Serum Levels Of Selective Inflammatory Cytokines And Nuclear Factor-κB Expression Of Heart In Reno-hypertensive Rats

Objective To evaluate the effects of angiotensin-converting enzyme inhibitor— Captopril and Chinese medicine—honghua extractives (HE) on the serum levels of selective inflammatory cytokines(IL-6, IL-10 and TNF-α) and nuclear factor-κB expression of heart in renohypertensive rats.Methods Using two-kidney-one-clip method, renal hypertensive rats (RHR) were obtained by liganting left kidney artery. 52 Wistar rats were randomized into four groups: sham-operation group (A), model control group(B), captopril group (C) and HE group(D).All rats were killed after being given the trial drugs 5 weeks, ELISA assays were used to detect the levels of IL-6 and IL-10, the levels of TNF-alpha were measured with radioimmunoassays, and the expression of NF- κB was measured by immunohistochemistry method.Results (1) compared with group B, the left ventricular hypertrophy was attenuated in group C, the ratio of left ventricle and body weight was 0. 00274±0. 00029(C) and 0.00310±0. 00012(B) respectively(p<0. 01), the levels of IL-6 and TNF-α decreased significantly(p<0. 01 and p<0. 05 respectively), immunohistochemistry staining showed the expression of NF-κB also decreased (p<0. 05), whereas the levels of IL-10 were not changed between the two groups(P>0. 05);(2) compared with group A, the ratio of left ventricle and body weight was 0.00274 ± 0.00029(0 and 0. 00262 ± 0. 00047 (A) respectively (p>0. 05), the levels of IL-6 increased significantly(p<0.01), and the levels of TNF-a unchanged(p>0. 05), immunohistochemistry staining showed the expression of NF-κB increased significantly (p<0. 01), whereas the levels of IL-10 were decreased significantly between the two groups(P<0. 05);(3) compared with group B, the left ventricular hypertrophy was attenuated...