Strains Differentiation And Research On Resistant Of Mycobacterium Tuberculosis By RAPD

Objective: Tuberculosis is an epidemic disease which treats people's health severely. Tuberculosis is of high incidence rate in China. About one hundred and thirty thousand deaths occur annually, two times of other epidemic diseases. The development of a reliable method to differentiate Mycobacterium tuberculosis isolates remains an important clinical goal, not only for the effective management of patients but also for understanding better the spread and evolution of this organism. Random amplified polymorphism DNA(RAPD) analysis is a rapid and simple DNA fingerprinting method that has already been used to fingerprint bacteria of medical interest. More recently, RAPD has been successfully applied to differentiate strains of M. tuberculosis . The present study is to report the use of RAPD analysis for the typing of strains of M. tuberculosis in Qingdao, and analyze the significant between drug-sensitive and drug-resistant strains, and to evaluate its clinical significance.Methods: 60 fingerprints of genomic DNA of M. tuberculosis strains, which were isolated from sputum specimens of 60 lung tuberculosis patients, were detected by RAPD. The productions of RAPD were analyzed by agarose gel electrophoresis. X2 analysis is used as the statistics method in this protocol.Results: Six types of DNA fingerprints were found and the main types were type I (31.7%), type II (30.0%), type III (26.7%). In those main type groups, the patients aged from 20 to 29, from 30 to 39 and from 40 to 49 were 34.0%, 20.8% and 22.6% respectively. For those main types, the distribution of the main drug-resistant strains showed significant difference compared with that of the drug-sensitive strains. The main drug-resistant strains were 52. 6% in type I , 77. 8% in type II and 75. 0% in type III. In the first treated patients and the retreated patients, the distribution of the main drug-resistant strains showed significant difference compared with that of the drug-sensitive strains.Conclusion: The study showed that RAPD analysis is a rapid, precise, sensitive,...