| OBJECTIVE 1. To explore the status of hepatitis B virus infection in the peripheral blood mononuclear cells (PBMC) of HBsAg positive pregnant women and their neonates. 2. To analyze the risk factors of the PBMC transportion from mother to fetus and the hepatitis B virus infection in PBMC. 3. To explore the role of PBMC in hepatitis B virus intrauterine transmission. METHODS pregnant women and neonates were collected between December,2001 and November,2004 in Taiyuan infectious hospital.The epidemiological base line data involving gestation and postpartum were also collected,maternal elbow vein blood and femoral vein blood from newborns within 24 hours were also collected at the same time. Following studies were performed: 1. HBV DNA in PBMC of HBsAg positive pregnant women and neonates were detected by ISH (In situ hybridization). 2. HBV DNA in PBMC smear of neonates in mother-fetus informative pairs (the GSTM1 gene of pregnant women was expression type and their neonates'was deletion type) were detected by DISH (double in situ hybridization). 3. All the data were analyzed by the statistical packages Visual FoxPor 6.0 and SPSS 10.0 for windows. 4. The risk factors of the PBMC transportion from mother to fetus and the hepatitis B virus infection in PBMC were analyzed by Logistic regression. RESULTS 1. The positive rate of HBV DNA in PBMC smear of 114 HBsAg positive pregnant women and neonates were 28.07﹪(32/114) and 32.46﹪(37/114) respectively. there was association between the HBV infection in PBMC of pregnant women and their newborns significantly (χ~2 =31.533,P﹤0.001). 2. There was 34 (62.96﹪) positive signal of GSTM1 gene of mother, 18 (33.33﹪) positive signal of HBV gene and 15 (27.78﹪) double positive signal of GSTM1 and HBV gene in PBMC smear of 54 newborns in mother-fetus informative pairs. 3. It shows there was association between the PBMC transportion from mother-fetus and HBV infection in PBMC of neonates (χ~2=9.957,P=0.002; OR=10.125 (2.027-50.583)). There was no association between the PBMC transportion and intrauterine infection (not including PBMC HBV DNA)(χ~2=1.518,P=0.218) and if PBMC HBV was involved in intrauterine infection there was significant association between the PBMC transportion and intrauterine infection (χ~2=7.183,P =0.007; OR=4.875(1.476-16.106)) by χ~2 test. 4. Risk factors of HBV infection in PBMC of newborns were only HBV infection in PBMC of pregnant women by Logistic regression (OR=12.413, 4.745-32.471), and there were no risk factors of the PBMC transportion from mother to fetus. CONCLUSION 1. As the HBV DNA was detected in PBMC smear of pregnant women and neonates, it shows that PBMC will be as the replicative and latent place of HBV, which will be great significance in chronicity, diagnosis and therapy of HBV. 2. There was association between the HBV infection in PBMC of pregnant women and the HBV infection in PBMC of newborns, and the former was the latter's risk factor. 3. There was no relation between PBMC transport from mother-fetus and HBV intrauterine infection (not including PBMC HBV), and no relation between PBMC transport from mother-fetus and placenta infection, it hints the path ofHBV intrauterine transmission caused by PBMC infected by HBV of pregnant women though the placental barrier was different from the path caused by placenta and serum, which was an important pathway of HBV intrauterine infection. 4. There was association between PBMC transport from mother-fetus and HBV intrauterine infection (including PBMC HBV), and the positive of HBV in PBMC of newborns could be as one of diagnosis marker of HBV intrauterine infection. 5. The DNA of mother was checked in PBMC of neonate shows that the PBMC of pregnant women have transported in blood circulation between pregnant woman and fetus. There was association between the PBMC transportion from mother-fetus and HBV infection in PBMC of newborns, it reveals the PBMC of HBsAg positive pregnant women which infected by HBV could cause HBV intrauterine transmission though cell conveying. |
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