Exression And Significance Of Cell Cycle And Apoptosis Associated Genes And Protein In Astrocytoma: Amplication Of Tissue Microarray Techinique

[Objectives] To investigate the protein expression and significance of cell cycle and apoptosis genes in astrocytoma on Tissue Microarray section. [Methods] Building a 204 spots tissue microarrayer and using immunohistochemcal S-P method to investigate the expression of P53, hTERT, Fas, ToPo II α, KI-67 and P16 in 56 astrocytomas and 10 nomal brain tissues. Statistic analysis was performed by SPSS10.0 software. [Results] 1. The expression of P53, KI-67, hTERT and ToPo II a protein was higher in astrocytoma than that in nomal brain tissue, the expression of these markers was obversely correlated to the grade of astrocytoma (P<0.05). The expression of Fas was higher in astrocytoma than that in nomal brain tissue, on the contrary, expression of P16 was lower in astrocytoma than that in nomal brain tissue. Expression of Fas and P16 showed no correlation to clinical stages. 2.The expression of P53 ,KI-67, Fas and hTERT protein had no significance with gender, age and size of tumor. The expression of P16 was higher among male than that among female(Z=2.050,P=0.004). The expression of Fas was higher among the group of older than that among younger(Z=2.628,P=0.009).3. The grading of astrocytoma was obversely correlated with the age of patient and KI-67LI in astrocytoma (F=3.997,P=0.025;F=22.07,P=0.000), but no correlation with the size of astrocytoma.4. The correlations of all these tagged proteins were as follows: these proteins showed significant obverse correlation including: ToPo II a and KI-67(p=0.552, p=0.025) ; hTERT and ToPo II a (r=0.416, p=0.001) ; ToPo II a and P53 (r=0.299, p=0.025) ; P53 and Fas (r=0.421,...